| Background and ObjectiveThyroid Carcinoma, one of the common malignant tumors of the endocrine system, has a rising incidence in recent years. It has become one of the important factors that threaten people’s health. Although there was a lot of research on thyroid carcinoma, and also have we made some achievements, but the diagnosis and treatment of thyroid carcinoma were not solved fundamentally.RAGE, specific receptors for advanced glycosylation end products, was the receptor of a variety of moleculars including advanced glycosylation end products (AGEs), beta amyloid peptide (A-β), high mobility group protein1(HMGB1), calgranulin (S100) and so on. RAGE and its many binding ligands activated various intracellular pathways through various channels and related mechanisms; they are involved in many kinds of pathology physiology and reproductive processes including the neuronal differentiation and regeneration, inflammatory reaction and so on. It was also found in recent years that it also had important impact on the invasion and metastasis of tumors. RECK (Reversion inducing cysteine rich protein with Kazal motifs) was latter found as a tumor suppressor gene. The studies showed that it expressed lower or missing in the tissues of laryngocarcinoma, esophagus squamous cancer, small cell lung cancer and other tumor tissues, the inaction of RECK gene may attribute to the invasion and metastasis of carcinoma.About the relationship between RAGE and RECK and the thyroid carcinoma, both domestic and abroad literatures have not yet been reported. In this study, the RAGE and RECK proteins were detected by immunohistochemical SP method in thyroid tissues, we also explored the relationship between the proteins and the thyroid disease (especially relations between the proteins and the thyroid carcinoma). The results might provide some theoretical basis for the pathogenesis, diagnosis and treatment of thyroid cancer.Methods1. The immunohistochemical SP method was used to detect the expressions of RAGE and RECK proteins in9cases of normal thyroid tissues,11cases of nodular goiter tissues,9cases of thyroid adenoma tissues, and69cases of thyroid carcinoma tissues, respectively.2. Statistical Analysis:all data was used SPSS17.0statistical package process, the comparison of the positive rate between groups was tested by chi-square (χ2), the expression strength of the proteins were checked by Wilcoxon rank sum test (two independent samples) and Kruskal Wallis H test (multiple orderly classification data). The correlation analysis of positive rates was based on the correlation of R X C contingency table analysis, and the correlation analysis of the expression strength was used Spearmen rank correlation analysis. The significance of the test standards was a=0.05.Results1. The positive expression rate of RAGE in normal thyroid tissues, nodular goiter tissues, thyroid adenoma tissues and thyroid carcinoma tissues was11.11%(1/9),18.18%(2/11),44.44%(4/9),89.86%(62/69), respectively, it showed a rising trend, the positive rate and expression strength in normal and benign thyroid tumor tissues were obviously lower than that in thyroid carcinoma tissues with statistically significant difference (P<0.05). In thyroid carcinoma tissues, the positive expression rate of RAGE in thyroid papillary carcinoma including papillary carcinoma tissues, follicular carcinoma tissues, medullary carcinoma tissues was:93.62%(44/47),85.71%(6/7),80.00%(12/15), respectively, but there was no statistically significant difference (P<0.05); the positive expression rate and expression strength of RAGE in thyroid tumor tissues had nothing to do with the patient’s age, gender and tumor size (P>0.05), but the expression strength associated with lymph node metastasis in patients (P<0.05).2. The positive expression rate of RECK in normal thyroid tissues, nodular goiter tissues, thyroid adenoma tissues and thyroid carcinoma tissues was11.11%(1/9),9.09%(1/11),33.33%(3/9),88.41%(61/69), respectively, it also showed a rising trend, the positive rate and expression strength in normal and benign thyroid tumor tissues were obviously lower than that in thyroid carcinoma tissues with statistically significant difference (P<0.05). In thyroid carcinoma tissues, the positive expression rate of RECK in thyroid papillary carcinoma including papillary carcinoma tissues, follicular carcinoma tissues, medullary carcinoma tissues was:93.62%(44/47),85.71%(6/7),73.33%(11/15), respectively, but there was no statistically significant difference (P<0.05); the positive expression rate and expression strength of RAGE in thyroid tumor tissues had nothing to do with the patient’s age, gendery tumor size and lymph node metastasis in patients (P<0.05)。3. In thyroid carcinoma tissues, the combination positive expression rate of RAGE and RECK proteins (RAGE+, RECK+) showed no correlation with patients’clinical pathological characteristics (P>0.05).4. In thyroid carcinoma tissues, the expression of RAGE was positively correlated with the expression of RECK (r=0.680, P=0.000).Conclusion1. The RAGE and RECK may be involved in the process of the occurrence of the thyroid cancers.2. The overexpression of RAGE protein may correlate with the process of lymph node metastasis of the thyroid cancers.3. The expression of RAGE gene positively correlated with the expression of RECK gene, these two genes may have a synergistic effect in the process of thyroid carcinoma. |
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