| Background and ObjectiveCervical cancer is one of the most common gynecologic malignancy, with aserious threat to women’s lives and health. With the advances in medical technology,molecular biology, tumor occurrence and development of deepening understandingmechanisms, gene therapy has become a new treatment after the traditional treatmentsuch as surgery, radiotherapy, chemotherapy. And the radiation-inducible genetherapy has been a new research in tumor therapy.Immediate early response gene-1(immediate early response geneX-1, IEX-1),belong to one family, immediate early gene (IEG), located in the short arm of humanchromosome6(6p21.3), expresses rapidly when been affected by growth factor,ultraviolet, ionizing radiation and ischemic hypoxia, and regulates cell growth,proliferation, differentiation or apoptosis. In different tissues overexpressed IEX-1impacts on cell positively or negatively. Study confirmed that the expression ofIEX-1mRNA increased significantly after by ultraviolet radiation, with a time-anddose-dependent manner. The inducecible by radiation makes IEX-1to become atarget gene and a potential clinical applications in cancer therapy.Studies confirm IEX-1expression reduced or disappeared in varieties of tumortissues, but its expression in cervical cancer is still reported rarely. In this study,analysis the radiation-induced of IEX-1in cervical cells by irradiation HeLa cells with ultraviolet, and use immunohistochemical method to detect in protein level theexpression and relationship of IEX-1, HIF-1α, NF-κB in cervical cancer, CIN andnormal cervical tissue， to further explore the link between IEX-1with thedevelopment of cervical cancer, to provide the basis for the clinical treatment ofcervical cancer as a target genes.Methods1． Using different doses of ultraviolet to irradiate HeLa human cervicalcarcinoma cells,after irradiation detect the expression of IEX-1mRNA by RT-PCR,and select the best UVB dose.2． Using the best doses of ultraviolet to irradiate HeLa human cervicalcarcinoma cells, detect the expression of IEX-1mRNA by RT-PCR at different timepoints after irradiation.3．Detect50cases of cervical cancer and40cases of CIN and26cases of normalcervical tissue by immunohistochemical SP method to analyzed the expression ofIEX-1, HIF-1α, NF-κB protein and their relationship.4． Applicate SPSS16.0software for statistical analysis, the formation ofmulti-measurement data were used to compare differences in the number ofsingle-factor analysis of variance. With α=0.05level for the test and P <0.05wasconsidered statistically significant.Results1．After irradiate HeLa human cervical carcinoma cells with different doses ofultraviolet, IEX-1mRNA expression were significantly increased (P <0.05).The bestUVB irradiation dose is in which IEX-1mRNA increased biggest.2．Using the best doses of40mJ/cm2ultraviolet to irradiate HeLa human cervicalcarcinoma cells, detect the expression of IEX-1mRNA at different cultured time afterirradiation, and the highest expression is found after cultured for4hours (P<0.05).3．The positive expression Rate of IEX-1in tissues of cervical cancer wassignificantly lower than those in tissues of CIN and normal cervix. The positive expression rate of HIF-1α and NF-κB in tissues of cervical cancer was significantlyhighly than those in tissues of CIN and normal cervix.4．In cervical cancer the expression of IEX-1,HIF-1α and NF-κB had nocorrelation with age,histological type and FIGO (P>0.05),but were related withhistological grade (P<0.05).5．A significant positive correlation was observed between the expression ofHIF-1α and NF-κB in cervical cancer (r=0.398,P<0.05),and a negative correlationwas observed between the expression of the former two and IEX-1(r1=-0.322,P<0.05；r2=-0.517,P<0.05).Conclusion1．IEX-1in HeLa human cervical cells has radiation-inducible, can be inducedby UVB, and has a time-and dose-dependent manner.2．IEX-1has low expression in cervical cancer, and regulate the growth andapoptosis of tumor cells with NF-κB and HIF-1α. |
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