Analyses For The Spectra Of Esophageal Cancer Blood

Esophageal cancer is one of the most common cancer in China. Especially in the western of our country, it has been one of the most cause of disease related death because of the unhealthy dietary style. Esophageal cancer has been a great threaten for the lives and health of the people. With the developing of spectroscopy, it has been more and more widely used in biomedicine, biophysics, chemistry, pharmacy, and so on. Especially in the detection of diseases, it attracts amounts of studies. Blood samples of healthy persons and esophageal cancer patients have been detected and analyzed by UV-visible absorption spectroscopy, regular Raman spectroscopy and surface enhanced Raman spectroscopy. The results demonstrate that the information showed by the UV-visible absorption spectroscopy is not enough relatively, and it is hard to distinguish the healthy and cancer blood samples. However, the Raman spectra have more advantages. In the Raman spectra of hemoglobin, it shows that the intense of the peaks at1562cm-1in the spectra of healthy hemoglobin are higher than the esophageal cancer hemoglobin, and the intense of peaks at1639cm-1are higher than1591cm-1in the spectra of healthy hemoglobin, while it is just the opposite in the spectra of esophageal cancer hemoglobin. This is attribute to the oxygen state of the hemoglobin, the unoxygen hemoglobin is in high spin state, the oxygen hemoglobin is in low spin state. It could be found that, comparing with normal persons, there are more iron ions in low spin state and less in high in the hemoglobin of esophageal cancer patients. It indicates that some iron ions in high spin state turned to low spin state because of cancerization, and this experimental result coincides with the fact that hemolysis is more likely to occur in cancer patients.Principal component analysis(PCA) and discriminate analysis is employed to statisticly analyze the result of the detection, it demonstrates that the overall diagnostic accuracy by UV-visible absorption spectroscopy, regular Raman spectroscopy and surface enhanced Raman spectroscopy are75.9%、92.7%及90.9%. It demonstrates that the Raman spectra show more special peaks than the UV-visible absorption spectra, thus the Raman spectra take more spectra information and show obvious advantage in the discrimination of healthy persons and esophageal cancer patients. The whole diagnostic accuracy of Raman spectroscopy are much higher than the UV-visible absorption spectroscopy. However, though the diagnostic accuracy of regular and surface enhanced Raman spectroscopy are comparative, comparing with regular Raman spectroscopy, the surface enhanced Raman spectroscopy(SERS) needs less sample and is more sensitive. Surface enhanced Raman spectroscopy(SERS) combined with statistic analysis is hopeful to be a new method for the diagnosis of esophageal cancer.