Study On Extract And Immune Activity Of Effective Principle From Artemisia Rupestirs L

Artemsia rupestris L. has been used as medicine for a long time in Xinjiang. Onthe basis of the previous study, Artemsia rupestris L. was further studied including theextract method, the determine of effective composition and the immune activity ofpolysaccharides, alcohol extraction from Artemsia rupestris L.The main research content and results were as follows:1.(1) Polysaccharides from Artemsia rupestris L. were extracted by ultrasonicextraction technologies. The optimized conditions were: the solid-liquid extractionratio was1:80, the extraction temperature65oC, and the extraction time60min.Under optimized conditions, the average extraction yield of polysaccharides fromArtemsia rupestris L. was32.32mg/g.(2) Polyphenols from Artemsia rupestris L.were extracted by ultrasonic extraction technologies, the optimum extraction solutionwas45%ethanol, the temperature of extraction was50oC, the solid-liquid ratio was1:60, and the time was60min. In this conditions, the average extraction percent ofpolyphenols from Artemsia rupestris L. was21.16mg/g.(3) Extraction of totalalkaloids from Artemisia rupestris L. was used two phase system assisted ultrasonic,the optimum extraction time was60min with propanol25mL and the ammoniumsulfate8.0g, the ratio of propanol to water was0.60:1. In this conditions, the averageextraction rate of total alkaloids from artemsia rupestris L. was0.436mg/g.(4) Amethod based on solid phase extraction (SPE) coupled with high performance liquidchromatography (HPLC) was developed for the simultaneous determination ofluteolin and quercetin in Artemisia rupestris L. The content of luteolin and quercetinin wild, cultivated Artemisia rupestris L. and different parts of cultivated Artemisiarupestris L. were compared. Chromatographic condition was set below: stationaryphase, C18(4.6mm×150mm,5μm) column; mobile phase, methanol-0.5%H3PO4(60:40, v/v), detected wavelength,360nm; column temperature,28oC; flow rate,1mL/min and injection volume10μL. Good linearities (r>0.9995) were reached for quercetin and luteolin in their respective areas of concentrations. The averagerecoveries was97.20%-102.60%, and the relative standard deviation (RSD) of1.17%-1.83%.2.The antioxidant activities of crude polysaccharides, polyphenols and totalalkaloids in Artemsia rupestris L. were evaluated by vitro hydroxyl radicals (·OH),super-oxideanion radicals(·O2-). The results show crude polysaccharides, polyphenolsand total alkaloids from Artemsia rupestris L. showed certain antioxidation capacities.3.The immune activity of polysaccharides, alcohol extraction from Artemsiarupestris L. were studied using the vitro cell test platform. The differentconcentrations of polysaccharides, alcohol extraction from Artemsia rupestris L.stimulated the bone marrow-derived dendritic cell (DC) of mice. With the increasingof concentrations of polysaccharides, the expression quantity of MHCII, CD86andCD40in surface molecule were increased. The alcohol extraction from Artemsiarupestris L. can stimulate CD40of DC. The polysaccharides, alcohol extraction havean evident effect on the mature of DC.4. The15trace elements in Artemsia rupestris L., including Li, B, V, Cr, Mn, Fe,Co, Ni, Cu, Zn, Mo, Cd, Tl, Pb and Bi, were analyzed by inductively coupled plasmamass spectrometry (ICP-MS) with microwave digestion mehtod. The results showedthat the recovery rates for all these elements were between80.06%and111.00%withRSDs less than5.00%. The Artemsia rupestris L. contains a variety of essential traceelements and harmful trace elements were relative low.