Flavored Four Inverse Scattering For Chronic Fatigue Syndrome Research Of Cellular Immune Mechanism Model In Rats

Objective:In this study, IL-2, IL-12, IFN-β, IFN-γ and IFN-α levels starting with changes in theperipheral blood of rats to zhongjing "Treatise on" Sini Guizhi, chronic Shichangpuintervention fatigue rat model to explore Jiaweisinisan cellular immune mechanismstreatment of chronic fatigue syndrome, which is JWSNS treatment of chronic fatiguesyndrome and provide experimental evidenceMethods:Choose SPF100health adult female Wistar rats,Weight (180±20)g, adaptive feedingone week, using weight stratified random number table experimental animals were dividedinto control group, model group, JWSNS group, hydrochloric paroxetine group,Xiaochaihutang group Siwutang group HuangqiJianzhong soup group, Chaihujialonggumulisoup group, ginseng decoction group, hyssop decoction group. In addition to the controlgroup, the other groups of rats with chronic unpredictable stress stimulate the manufacture ofanimal models of moderate intensity, of35days, the stress method comprising: exhaustiveswimming (19℃, depth40cm), bound (bondage homemade bottle continued5h), waterdeprivation fasting24h, sleep deprivation24h, clip the tail continued to1min. Weighedevery seven days, and changes to Open-field detection of ethology. After the model, thecontrol group and model group were given distilled water by gavage1.0ml/100g othergroups by gavage, the dose as follows: JWSNS5.93g/kg, paroxetine hydrochloride20mgparoxetine hydrochloride in tablets dissolved in94ml saline Xiaochaihutang8.47g/kg,Siwutang4.23g/kg, HuangqiJianzhong soup10.48g/kg, Chaihujialonggumuli soup7.41g/kg,ginseng decoction1.06g/kg, hyssop decoction1.59g/kg, the weight of each group are 1.0ml/100g administered once/day, administered21days after the last administration butwater, fasting24h, drawn. Enzyme-linked immunosorbent assay (ELISA) was measuredIL-2, IFN-α, IFN-β, changes in IL-12, IFN-γ levels in rat serum.Results:Compared with the control group, modeling modeling rats in each group during theweight loss, Open-field experiment horizontal movement, vertical movement scoredecreased; model group, serum IL-2, IL-12, IFN-β, IFN-content decreased γ, IFN-α levelsincreased. After treatment, JWSNS group, paroxetine hydrochloride group Xiaochaihutang,Chaihujialonggumuli soup group, Siwutang group, hyssop decoction group, ginsengdecoction group and serum HuangqiJianzhong soup group IL-2, IL-12, IFN-β, IFN-γ levelsincreased, IFN-α was decreased.Conclusion:A chronic unpredictable moderate compound stimuli can induce experimental animalmodel of chronic fatigue syndrome.2JWSNS, ginseng, and HuangqiJianzhong soup can beincreased serum IL-2, IL-12, IFN-β, IFN-γ levels, lower serum IFN-α levels.3Shu Qimachine method, warming the gas law, added strength and effective treatment of chronicfatigue syndrome. Its therapeutic mechanism may regulate the body’s cellular immunefunction.