Research On The Constituents And Their Anti-inflammatory Of Gentiana Triflora

Gentiana triflora Pall.(Gentianaceae) is widely used to treat inflammation, jaundice, hepatitis and rheumatism.In this study, we explored the active constituents of Gentiana triflora. The main innovation and academic contributions of this thesis are presented as follows:(1) Column chromatography was used to separate constituents from the dichloromethane portion in80%ethanol extract of Gentiana triflora. Three new compounds including a benzo seven membered ring compound gentioxepine (1), two secoiridoid glucosides:(1S,5R,9R)-deglucosyltrifloroside2) and (1S,5R,9R)-scabraside (3), together with seven known ones:(+)-syringaresinol (4), deglucogelidoside (5),3,4-dihydro-1H,6H,8H-naphtho [1,2-c:4,5-c’,d’] dipyrano-1,8-dione (6), deglucoscabraside (7),2-hydroxy-3-O-β-D-glucosyloxy benzoic acid methyl ester (8), gentiolactone (9), and trifloroside (10), were isolated from ethanol extract of Gentiana triflora Pall.. Their structures were mainly confirmed on the basis of NMR, MS, IR, CD, and UV spectral evidences.(2) HPLC-ESI-MS was used to study fragmentation paterns of secoiridoids. The fragmentation patterns were applied to clarify constituents in the extract of Gentiana triflora.(3) Anti-inflammatory activities of eight compounds isolated, as well as gentiopicroside, were evaluated in the macrophage cell line RAW264.7. The results showed that the three new compounds and compound7could significantly suppressed lipopolysaccharide (LPS)-induced production of NO, with IC502.2μM,37.5μM,17.6μM and6.9μM, respectively. Among them, compound1,3and7showed stronger inhibitory activity than that of a clinically used drug indomethacin. Other tested compounds exerted moderate inhibiting activities.