The Effect Of IL-10and TNF-α On Mice Hippocampal Neurons In Hepatic Encephalopathy Induced By Alcohol And Carbon-tetrachloride

Objective:To explore the effect of IL-10and TNF-α on hippocampal neurons in hepatic encephalopathy induced by alcohol and carbon-tetrachloride.Methods:Forty mice from three types were divided into four groups:Normal group(ten wild type mice),model one group(ten wild type mice),model two group(ten IL-10knockout mice)and model three group(ten TNF-a knockout mice). All mice from model groups were fed ethanol diet and intraperitoneal injection of CCl4to establish a mouse model of chronic liver failure-caused hepatic encephalopathy. In order to estimate whether the model was established successfully, the behavior activities of mice were observed, Morris water maze test was taken, the concentration of the serum ALT and AST was measured by extracting the eyeball blood, and the pathological changes in live were observed. The expression of GRP78in the hippocampal neurons was detected by immunohistochemistry. Finally, using an optical microscopy and electron microscopy to observe the pathological changes in the brain and liver of mice.Result:(1)The degree of injury for learning and memory in all model group mice were most significantly when compared with the Normal group. The model2group showed that the escape latency was longer than model1group, but it is not significantly.The escape latency of model3group was significantly shorter in Morris water maze test, when compared with model1group(P>0.05).(2)After modeled6weeks, levels of blood ALT, AST in model groups significantly increased compared with Normal group.(3) Varying degree of liver impairment presented in all mice from3model groups.(4)Through the optical microscopy and electron microscopy observation, the damage of hippocampal neuron in model2group was more serious than model1group, the number of neurons was decreased, the immunohistochemical figures showed the disordered arrangement of neurons.The damage of hippocampal neuron in model3group was not very severe when compared with model1group.Conclusion:The mouse model of chronic liver failure-caused hepatic encephalopathy was established successfully. In model2group, the damage of hippocampal neuron was more severe, it suggests that Interleukin-10may play an protective role in hepatic encephalopathy while TNF-a has significant damage effect.