| Objective:Sepsis model was induced by cecum ligation perforation(CLP) in sepsis rats. Use of gene microarray technology to detect the liver gene expression difference of the sepsis rats treated with "Jun Du Yan Bingzhi" and further explore the the internal mechanism of the sepsis rats’liver gene expression regulated by "Jun Du Yan Bingzhi". We explore the mechanism of this disease in gene level to provide a new idea for curing sepsis.Mothods:90Wistar rats were divided into control group (30)%Sepsis model group (30) and "Jun Du Yan Bingzhi" group (30)ramdomly. Sepsis model was established by the cecum ligation perforation method in the experimental group. After model was made up, sepsis model group was treated with0.9%NS10ml/kg by intraperitoneal injection;"Jun Du Yan Bingzhi" group was treated with Bid with antibiotics (tienam0.lg/kg) and Xuebijing injection10ml/kg by intraperitoneal injection,"Xuebijing2"(self-made complex prescription apozema of the traditional Chinese medicine)3ml/100g (density:3g/ml) by intragastric administration. Obverse the survival status of the rats after the perforation, and analyze the48h and72h survival rates of the rats. All the rats would be put to death after72h and collected the right upper lobe hepatic tissue, which is preserved in liquid nitrogen in asepsis condition. Extract the total RNA with Trizol from the rats’ liver tissue and prepare gene chip. Gene expression difference of rats’liver tissue from sepsis model group/control group,"Jun Du Yan Bingzhi" group/sepsis model group was detected. The ratio of Cy3/Cy5>2.0or<0.5was used to screen differential genes, and NCBI database was used to identity the function of differential genes.Results:1. The48h and72h survival rats of rats in "Jun Du Yan Bingzhi" group (90%and63.3%, respectively) were significantly higher than that of the sepsis model group (26.7%and13.3%, respectively).2.281and482differential genes were found in Sepsis model froup and "Jun Du Yan Bingzhi" group, respectively.146genes were up-regulated and135genes were down-regulated in the sepsis model group;281genes were up-regulated and201genes were down-regulated in the "Jun Du Yan Bingzhi" group. Differential expressed genes involves in cell apoptosis, proliferation, differentiation, growth, signal pathways, immune response, inflammation, protein transport/processing/modify/degradation.Conclusion:1."Jun Du Yan Bingzhi" has significant therapeutic effect on sepsis rats.2. Plenty genes were out of regulated in liver tissue of the sepsis rats, such as genes regulated in cell apoptosis, proliferation, differentiation, growth, signal pathways, immune response, inflammation, protein transport/processing/modify/degradation. That means the effect of sepsis on rats gene expression is multiple. Accurate and quick detection of the abnormal gene expression has great meaning for sepsis therapy.3."Jun Du Yan Bingzhi" regulate the abnormal expressed gene in sepsis rats, which can modulate the most differentially expressed liver genes happen returned to normal, and significantly increase the survival time of the sepsis rats and decrease the48h and72h death reats. |
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