Effect Of Mycobacterium Tuberculosis On B Cell Development And Differentiation And T Cell Subsets In BALB/c Mice

ObjectivesTuberculosis is a major infectious disease hazards to human health.A largenumber of data indicate that anti-TB immunity mainly innate immunity andcell-mediated immunity, but recent studies have shown a protective effect of B cellresponses in tuberculosis, while B cells in tuberculosis in response subsets changesare unclear. This paper intends to use a mouse model of Mycobacterium tuberculosisinfection on understand of B lymphocyte development and differentiation of T cellsubsets and functional subsets of Th1/Th2.Methods1Establishing the infection model of Mtb: Take5-6weeks old BALB/c mice,female, into Dali isolates group, H37Ra infection group and the saline group. Weinjected the bacteria with106CFU in per mouse. When mice infected4weeks or8weeks, they were sacrificed. Taking the lung, liver and kidney of the mouse,bacterial cultured and pathological changes observed.2Testing the development and differentiation of B cell and the subsets of T cell inmice infected with Mtb: Made the cell suspensions with the bone marrow, spleenand lymph node, anti-mouse antibody labeled with a different fluorescent.Analysis the results use the FACSCalibor.Results1TB infection can affect the development of B cell differentiation, induce differentiation of B cells into mature cells, B cells during early development(pro-B, immature B and T1B cells) reduced the increase of mature B cells andcan induce the activation of B cells and memory cells increased, so that the bodyis more conducive to anti-tuberculosis infection.2The early infected of H37Ra and Mtb in mouse, CD3+T cells and CD4+T cells werereduced in the spleen, and CD8+T cells were increased. Cell-mediated immunityissuppressed, In the advanced stage of infection it returned to normal. It’s no effect onCD3+T cells and the subsets of T cells in lymph node; in late of Mtb infection, Th1response appeared, but there is no significant of Th2response.ConclusionsPreliminary studies have shown H37Ra and Mtb clinical isolates in the B cellsubpopulations, B cell development and differentiation of T cell subsets was nosignificant difference in the distribution of influence, which in the past only tounderstand H37Ra reduced virulence, but retains immunogenicity conclusion isconsistent also explains Mtb Dali immunogenicity and clinical isolates ofMycobacterium tuberculosis immunogenic roughly the same.