| Background:Spinal Ossifieation of Ligamentum Flavum (OLF) was reported by Polgar as early as1920, and Yamaguchi et al reported thoracic myelopathy caused by OLF in1964. From then, researches of thoracic OLF get more and more attention by scholars from various countries. Each segment of the spine, from cervical to lumbar, may suffer from OLF, especially the lower thoracic thoracic is the most common. Thoracic OLF is one of the main reasons leading to thoracic spinal stenosis, and it is often associated with other topical or systemic diseases, which make clinical manifestations complex and diverse. Consequently, it is difficult for the diagnosis and treatment of the disease. OLF is very common in Japanese from50to70years old, especially having a higher incidence in these patients, who were diagnosed as diffuse idiopathic skeletal hyperostosis (DISH), skeletal fluorosis, diabetes and ankylosing spondylitis (AS). Although lots of basic researches and clinical investigations are devoted to the cause of OLF, the etiology and pathological mechanisms of OLF are still remain unclear.In recent years, MicroRNAs (miRNAs) regulating the gene transcription becomes a hot topic in variey of disciplines. Many studies reported that miRNA played an important role in the bone development and formation. MicroRNAs are very short length of non-coding RNA, by reducing the stability of target genes or inhibit its translation level to influence cell differentiation, proliferation, and apoptosis. There are many miRNAs stably existing in human serum, and expression of miRNAs is consistent with normal population. It has been shown that serum miRNAs could change specifically, when body suffers from disease. Specific miRNAs of abnormal expression are related to the abnormal metabolism. It helps to understanding the pathogenesis, early diagnosis and prognosis of disease.Studies have shown that patients are more likely to suffering from OLF diagnosing as obesity, diabetes, hyperinsulinemia, and calcium and phosphorus metabolism disorders, suggesting abnormal bone metabolism in these patients. Until now there has been no study on miRNAs in serum of patients with OLF. Objective1. To detect differential expressed microRNAs in serum between CS and contral.2. To analyze the differential expressed microRNAs with bioinformatics analysis.3. To find key microRNA for further research through comparative analysis combined with bioinformatic prediction.Methods1. Serum was collected from3patients with formation failure,3patients with segmentation failure and3age-and gender-matched normal persons. Then total RNA was isolated and tested with Exiqon miRNA Arrays. The slides were scanned and followed by data extraction. After statistical analysis we got the list of differential expressed microRNAs.2. Target gene and pathway prediction was made.3. The key miRNA was selected after comparative analysis combined with bioinformatic prediction.Results1. A list of differential expressed microRNAs was made by setting the hreshold of up/down regulated microRNAs as Foldchange>=2and P value<0.05.20up-regulated miRNAs and3down-regulated miRNAs were detected in the OLF group VS control group.2. The key miRNAs were selected. For the OLF,3up-regulated:has-miR-765、 has-miR-56、hsa-miR-205-5p.Conclusions1. This is the first time to study comparative serum miRNA of OLF. And differential regulated miRNAs in serum were detected between patients and normal persons.2. The key miRNAs of the group of OLF patients were selected for the following research. |
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