| Objective WIF-1(Wnt inhibitory factor-1) was one of the most common antagonists of wnt signal pathway,this experiment was to study the expression of β-catenin interfered with WIF-1in human osteosarcoma MG-63cells.Methods Human MG-63cells interfered with WIF-1in the concentration of Ong/ml、48ng/ml、60ng/ml、80ng/ml、120ng/ml)was cultured, the concentration of Ong/ml was considered as the blank group.The expression of β-catenin was retrospectively detected by the methods of real-time fluorescent quantitative polymerase chain reaction(FQ-PCR) and Western-Blot at the genetic and protein level. Culturing Human MG-63cells,interfered with the same concentration of WIF-1, the quantity of cells was partly noted in the hour of0、12、24、8、2、96h. The number was compared between in the blank group and experiment group.Results interfered with different concentrations(Ong/ml、48ng/ml、60ng/ml、80ng/ml、120ng/ml) of WIF-1,It was showed by FQ-PCR that the relative fold-changes of P-catenin mRNA was retrospectively recorded as1、0.55±0.30、0.44±0.24、0.35±0.15.0.24±0.21. compared with the blank group, the stimulating groups were all found to have a lower expression(F=6.02, P<0.05). with the improvement of concentrations of WIF-1,the expressions of β-catenin presents a decreasing trend.However, there was no significant differences in the stimulating groups themselves(P>0.05); Western-Blotting showed us that the relative fold-changes of P-catenin/β-actin was separately memorized as1.4±0.11、0.86±0.10、0.51±0.11、0.45±0.06、0.18±0.03; compared with the blank group, differences are statistically significant (F=329.5, P<0.05). compared with the stimulating group,the blank group was found to have a higher proliferation.Conclusion WIF-1could decrease the expressions of β-catenin in the canonical wnt signal pathway of human osteosarcoma MG-63cells and inhibit the proliferation of human osteosarcoma MG-63cell, wnt/β-catenin signal has the important control action in MG-63cell line. |
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