| Objective:To investigate the effects and the mechanisms of Yulangsan aqueous extract and polysaccharide on chronic hepatic injure in rat induced by alcohol and its mechanism.Methods:The model of rats with chronic alcoholic hepatic injury was intragastrically perfused with high concentration alcohol once a day, and the Yulangsan aqueous extract and polysaccharide were synchronously given once a day. After24weeks, all rats were killed and dealt with as follow:(1) the serum and hepatic tissue were taken, and the activities or content of ALT, AST, SOD, MDA, GSH, GSH-PX, ADH and ALDH in the serum and hepatic tissue were measured.(2) The hepatic tissue containing3cm×3cm×5cm volume in each rat was obtained and then fixed in10%neutral formalin solution. After24hours, each tissue was paraffin-embedded, hematoxylineosin (HE) and immunohistochemistry stained.(3) The expressions of β-Catenin, mTOR, p70S6K, C-myc, CyclinD, Col-I and GSK-3β mRNA in the hepatic tissue were detected by Q-PCR. The expressions of β-Catenin protein were detected by immunohistochemistry. Results:Compared with the model group, the levels of MDA in the serum and tissue of treatment groups were reduced significantly (P<0.01), while the GSH contents in those of treatment groups were obviously increased (P<0.01). The activities of ALT and AST decreased significantly (P<0.01) and the activities of SOD, GSH-PX, ADH and ALDH etc. increased significantly (P<0.05). The condition of liver injure in rats was effectively alleviated. β-Catenin protein levels decreased compared with the model group. The expressions of P-Catenin, C-myc, CyclinDl, Col-I, GSK-3β mRNA were significantly reduced (P<0.05).Conclusions:High dosage of Yulangsan polysaccharide and aqueous extract has the protective effect on the alcohol-induced liver injury in rats though the mechanism that may be related to removing free radicals and inhibiting the lipid peroxidation as well as reducing the level of gene expressions. |
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