Experimental Studies Of Hemodilution Effect On The Spinal Cord Function

Objective：To observe the changes of somatosensory evoked potentials, motorevoked potentials and spinal cord function after normovolemic hemodilution, Toexplore perioperative hemodilution effect on spinal cord function.Methods：20healthy male SD rats weighing359±5.12g, hemoglobin144±13.5g/L were randomly divided into two groups (n=10rats): hemodilution group (groupA) and control group (group B). We performed BBB spinal movement function scoreand internal jugular vein catheterization for these two group rats. The rats in group Awere given hemodilution treatment with6%hydroxyethyl starch one weeklater. Atthe same time we monitored the changes of heart rate, blood pressure, hemoglobin,amplitude and incubation period and morphological changes of somatosensory evokedpotentials and morphological changes of motor evoked potentials. Until thehemoglobin to reach50±5g/L, three hours later performed spinal cord BBB motorfunction score again, took spinal perform perfusion fixed HE dyeing again, observedthe morphological changes under light microscopy. Group B monitoring the same asgroup A monitoring point were before the trial, no treatment and waiting for40minutes, and three hours respectively, we processed spinal cord BBB movementfunction score, and then undergo spinal cord HE staining after anesthesia perfusionfixation and observed the morphological changes under light microscopy. The relateddata were performed statistical analysis.Results： Comparison before and after hemodilution in group A, hemoglobinlevels were significantly lower, The latency period of SSEP was prolonged (P=0.002,P<0.05).There was significant difference. The amplitude volatility was decreased(P=0.734, P>0.05). Comparison group A and group B, the BBB movement behaviorscore at3hours after hemodilution shew no significant difference.(P>0.05). Themorphology of spinal cord neurological nuclear also has no difference under lightmicroscopy examination useing HE staining.Conclusions：Decreased hemoglobin caused by hemodilution will have an impact on spinal cord nerve conduction. Clinically to maintain good spinal cord hemoglobinlevels for spinal related surgery conducive to the maintenance of conduction function.