Study On Synthesis、Characterization And Antitumor Activity Of Rare Earth Metal Complexes With All Trans Retinoic Acid And Amino Acids

Cancer has been a serious threat to people’s health, but also the main burden of globaldisease treatment. Human against tumor diseases that would become dated back to2000yearsago, almost50years, with the progress of medical research, people have the fundamentalknowledge of tumor change, for tumor prevention and treatment has become the focus ofpeople from all walks of life. For inorganic drug researchers, an important part of thechallenge is how to better balance the metal complexes of pharmacological activity andpotential toxicity, thus to realize the reasonable design of metal complexes.This paper reports the rare earth ions (Nd3+, Eu3+and La3+, Sc3+)-all trans retinoicacid-amino acids(L-arginine, L-valine, L-glutamate) ternary complexes of synthesis,characterization and its anti-tumor activity in vitro experimental results. The results show thatthe basic stronger antitumor activity of the complexes of ligand all trans retinoic acid, threecorresponding amino acid and metal nitrate，rare earth complexes on three kinds of cancercells(HepG2﹑A549﹑Hela) growth inhibition basically increases with the concentration ofcomplexes and enhancement. The interaction of these complexes with DNA was studied byfluorescence spectra, viscosity and UV spectra, and the results showed that the type ofinteraction is mainly intercalation, which may be the one of reasons for antitumor activity ofthe complexes. The research of this paper provides certain ideas and experimental basis todesign efficient and low toxicity of rare earth antitumor drugs.This paper is divided into the following five parts:1. Reviewed the research progress of the antitumor drugs. Focused on the metal and itscomplexes, retinoic acid, amino acids, and the structure characteristics and properties of rareearth elements, physical activity and its research status in antitumor; Introduces the interactionbetween metal and DNApatterns, commonly used methods for antitumor drug screening.2. Four complexes of REL2L′Cl·nH2O(RE:Nd3+、Eu3+、La3+、Sc3+; L=all-trans retinoic acid;L′=L-arginine acid ion) were synthesized. The compositions of complexes were tested by IR,UV, fluorescence spectra, elemental analysis and TG-DTA methods. Meanwhile, theantitumor activity against HepG2、A549and Hela in vitro growth cells was investigated byMTT test method. The interaction of these complexes with DNA was studied by fluorescencespectra, viscosity and UV spectra,The experimental results show that the ScL2L’Cl complexeshave great potential drug use value. 3. Four complexes of REL2L′Cl·nH2O(RE:Nd3+、Eu3+、La3+、Sc3+; L=all-trans retinoic acid;L′=L-valine) were synthesized. The compositions of complexes were tested by IR, UV,fluorescence spectra, elemental analysis and TG-DTA methods. Meanwhile, the antitumoractivity against HepG2、A549and Hela in vitro growth cells was investigated by MTT testmethod. The interaction of these complexes with DNA was studied by fluorescence spectra,viscosity and UV spectra, The experimental results show that the EuL2L ’and ScL2L’complexes on the anti-tumor drugs research and development has good research prospect.4. Four complexes of REL2L′Cl·nH2O(RE:Nd3+、Eu3+、La3+、Sc3+; L=all-trans retinoic acid;L′=L-glutamate) were synthesized. The compositions of complexes were tested by IR, UV,fluorescence spectra, elemental analysis and TG-DTA methods. Meanwhile, the antitumoractivity against HepG2、A549and Hela in vitro growth cells was investigated by MTT testmethod. The interaction of these complexes with DNA was studied by fluorescence spectra,viscosity and UV spectra, The experimental results show that the EuL2L ’ is a kind of potentialanticancer drugs, and a more in-depth studies are needed to determine its medicinal value.5. Reported that the four kinds of rare earth oxides (Nd2O3, Eu2O3, La2O3, Sc2O3) of theantitumor activity against HepG2in vitro growth cells. Further, from the angle of rare earthions, rare earth ternary complexes of the antitumor activity against HepG2、A549and Hela invitro growth cells are summarized, in order to provide certain experiments in anti-tumor drugsresearch.