Chlorogenic Acid Attenuates Intestinal Barrier Damage In Colitis Rats

Intestinal mucosal barrier is composed of mechanical barrier, biological barrier,chemical barriers and immune barrier. The structural basis of mechanical barrier isintact junctions between intestinal epithelial cells. Many studies have shown thatmechanical barrier dysfunction may precede clinical evidence of IBD. The restrationof intestinal barrier function is conducive to the ease of inflammation and immuneresponses and the improvement of intestinal permeability. This research aims toexplore the effect of chlorogenic acid (CGA) on the mechanical barrier of the colitisrats induced by TNBS.This experiment was to explore of the effect of chlorogenic acid in the intestinalbarrier function of the colitis rats induced by TNBS.32healthy SD rats were chosenand were randomly divided into four groups according to the weight: control group,CGA group (to fill the stomach every day60mg/Kg), TNBS group (enema a100mg/Kg) and CGA+TNBS group (to fill the stomach every day60mg/Kg of CGA+enema100mg/Kg TNBS). Each group has eight rats. Test cycle is4weeks. Thisexperiment studied the effect of CGA in colitis rats’ intestinal barrier function: growthperformance, intestinal morphology, the change of the distribution and expression ofthe tight junction protein. The expression and distribution of MLCK also was studiedto determine whether the work of CGA was managed by MLCK.The results show that:(1) CGA can increase the weight, feed intake gain, and theorgan index, and decrease the disease activity index;(2) CGA can signific antlyelevate the activity of alkaline phosphates, lactate dehydrogenase, and myelope-roxidase;(3) According to the picture of colonic appearance, HE staining andtransmission electron microscopy, we found that chlorogenic acid can reduce theinjury of colitis rats’colon;(4) CGA can significantly reduce the L/M of the urine, thecontent of serum endotoxin;(5) CGA can significantly increase the expression oftight junction protein occludin, ZO-1, claudin-1in colitis rats;(6) CGA couldsignificantly inhibit the expression of MLCK and increase the fibrous actin (F-actin)expression. Above all, CGA can alleviate the injury of intestinal structure and function,reduce intestinal permeability, and increase the expression of the tight junctionprotein.