Effects Of Jiashenlingzhi On Sleep And The Immune Function In Mice

Determine the Jiashenlingzhi’s health benefit: improve sleep, regulate immune system.Provide a pharmacological basis for the development of commercial product.Healthy ICR mice randomly divided into control group,500、1000mg/kg of aqueousextracts from Jiashenlingzhi group,500、1000mg/kg of ethanol extract from Jiashenlingzhigroup and0.25mg/kg does of Estazolam group. Intragastric administration once a day for30days. Experiments of direct sleep-induced test, locomotor activity, measure time for sleepinduced by pentobarbital sodium. measure mice sleeping rate under the threshold hypnogenesisdosage of pentobarbital sodium and affecting sleeping delitescence induced by tobarbitalsodium.Healthy ICR mice randomly divided into control group and500、1000mg/kg of ethanolextract from Jiashenlingzhi group. Intragastriced administration once a day for30days.Determined the GABA,5-HT, NA, DA content in mice brain to explore the mechanism ofcounterpart on improving sleep. The does500、1000mg/kg of ethanol extract fromJiashenlingzhi could dependent manner increase GABA levels in mice brain, but had no effecton NA、5-HT nor DA.Healthy ICR mice randomly divided into control group,500、1000mg/kg of aqueousextracts from Jiashenlingzhi group and500、1000mg/kg of ethanol extract from Jiashenlingzhigroup. Intragastriced administration once a day for30days. Laboratory test on regulating theimmune system: compare the murine thymus and spleen coefficients, study mice carbonclearance capacity, comparative transformation of T-lymphocytes, compare NK cell cytotoxicityand analysis the haemolysin level in mice serum haemolysin level in mice serum.Ethanol extractfrom Jiashenlingzhi could increase the spleen coefficient, and1000mg/kg dose of thecompound could increase the thymus coefficient of mice, ethanol extract from Jiashenlingzhiand1000mg/kg aqueous extracts from Jiashenlingzhi could increased T lymphocytetransformation in vitro,500、1000mg/kg of ethanol extract from Jiashenlingzhi effectivelyenhance the ability of NK cells,1000mg/kg dose of Jiashenlingzhi could increase thehaemolysin level in mice serum, but it doesn’t have effect on phagocytic index A or clearanceindex K.Ethanol extract from Jiashenlingzhi could improve the sleep function with improve thecontent of GABA. Aqueous extracts from Jiashenlingzhi have no function on sleep of mice.Ethanol extract from Jiashenlingzhi could improve immune function of mice, Ethanolextract from Jiashenlingzhi compared Aqueous extracts from Jiashenlingzhi with bettereffect.Ethanol extract from Jiashenlingzhi was the best prescription.