| Wound healing is the repairing process after skin tissue injury and reduction, it refersto the regeneration of blood vessels and nervous tissue, newborn of granulation tissues, theformation of scar tissue in trauma parts and a series of complicated processes, all aboveparts of cross function effect at the same time, affecting the whole repair process of tissuetrauma. Tissue wound healing problem is also a clinical common disease, it bringsextremely serious impact to the patient’s physical and mental health. In the meantime, thepopulation of diabetics in our country has increased dramatically in recent years, and thediabetic wound healing is out of order(such as severe cases of diabetic foot can causetissue edema, ulcers and necrosis)—the tissue repair of diabetes will damage at variousstages, including abnormal inflammation, microcirculation block, delay in epithelial tissueand blood vessel formation and blockage of collagen accumulation etc—So the problem of how to fix the wound healing of diabetic patients is becoming a more serious clinicalchallenge. In conclusion，developing much more efficient, secure and economic treatmentto accelerate wound healing(especially the wound healing of diabetic patients) and to easethe pain of patients, will improve patient’s quality of life great significantly andmeanwhile have’ very important social and economic benefits.The biological effect of electromagnetic field is a hot spot in the field of BiomedicalMedical Engineering research in recent years, based on the different generated methods ofelectromagnetic field, which can be divided into the biological effect of static magneticfield，the biological effect of alternating magnetic field and pulse electromagnetic field.Generally speaking, steady magnetic field can be obtained by steady disk(such asneodymium iron boron disk), by virtue of its more simpler, such as portability and securityadvantages, welcomed by the majority of scientific researchers and clinical workers, andits biological effects also have been more extensively researched. Many studies haveconfirmed that static magnetic field can significantly improve the microcirculation of thebody, has obvious anti-inflammatory and analgesic effect, meanwhile, magnetic fieldwithin a certain intensity can improve the body’s bone quality, regulate bone metabolism,and can inhibit the growth of tumor cells to some extent. However, the static magneticfield for the wound healing of tissue, especially the diabetic wound healing, very littleresearch at home and abroad. At the same time, the static magnetic is how to improve thetissue’s wound healing process, how are its effects, what are the mechanisms of its effectis still unknown. Therefore, in this study we first through the way of combining theoreticalcalculation and actual measurement to analysis and compare the space and timedistribution characteristics of magnetic fields generated by the rubidium iron borondisk;then, through creating skin wounds model in the back of rats, useing static magneticfield to stimulate the trauma part, evaluating the tissue’s wound healing effects from thewound healing rate and total healing time, histological study and wound tensile strength.Third, diabetic rats was induced by intraperitoneal injection of Streptozocin, the rats’wound in back was created, static magnetic field to stimulate, evaluation was carried outon the wound healing condition. With the advantage of noninvasive and economic aspects of static magnetic field, this study provide some valuable theoretical and experimentalbasis for clinical application in future.The main work of this subject is as follows:First, the static magnetic field was produced by neodymium iron boron disk withpermanent magnet as the basis magnetic field of study, and use of Comsol Multiphysicssoftware for3-d simulation on static magnetic field produced by disk to build spatialdistribution model of static magnetic field to make up for the inadequacy of abstract andfuzzy situation in static magnetic field’s distribution. Through the analysis andmeasurement,180mT static magnetic field required in later experiment was created by theapplication of permanent magnetic disk, spatial distribution of the static magnetic intensitywas measured by Gauss Meter, through the numerical analysis method to analyze andcompare the actual measurement data and the simulation data, the results show that bothprecise match. It provided a standardized model for3-d spatial distribution of one kind ofstatic magnetic field, and provide a specific demand of static magnetic field forexperimental research platform, in the case of experimental data accuracy less demanding,relevant data can be used as theoretical derivation and calculation.Second, research on the therapeutic effect of180mT static magnetic field on skintissue wound in back of normal Sprage-Dawley rats.24male3months oldSprage-Dawley rats, randomly divided into wound group and static magnetic fieldtreatment group, the number of each group is12. One open circular wound with1.5-cmdiameter in the dorsum was created on either normal mice, and dressed with hydrocolloid,N pole of static magnetic disks fixed to the hydrocolloid dressing with double-sidedadhesive in the magnetic field group I. The rats subjected to static magnetic field treatmentgroup were exposed to180mT static magnetic field. On days5,12and19, four rats ineach group were euthanized, and the gross wound area, the histological analysis and thetensile strength of the soft tissue were respectively qualified and compared. Dates of everygroup were obtained through photography during the wound area measurement, and werecalculated and compared by the wound healing rate and total healing time. Histologicstudy of wound tissue was processed by formalin fixation and paraffin section, HE staining, the details of each tissue repair were observed and compared by microscope, theresults show that the static magnetic field obviously improved the skin wound healing rateand shorten the healing time in rats in5,12, and19days. Tensile strength is measured byintensity measuring instrument, the results show that, compared with the wound group thetensile strength levels in the static magnetic group were significantly higher, the numberand quality of collagen deposition in trauma were significantly increased. Our resultssuggest that180mT static magnetic field has good therapeutic effect on wound healing inrats.Third, in this study, we aim to systematically evaluate the therapeutic effects of180-mT moderate intensity gradient static magnetic field on diabetes-induced epidermalwound healing in rats, and thus provide adequate scientific basis for the application ofstatic magnetic field in clinics for diabetic wound healing.48male three months oldSprage-Dawley rats was given a single intravenous administration of streptozotocin at adose of50mg/kg versus body weight. Mice with fasting blood glucose concentrationhigher than16.7mmol/L were used in the experiment. Sixteen normal mice were used asthe Control group, and32diabetic mice were randomly divided into Diabetes or staticmagnetic field treatment group. One open circular wound with1.5-cm diameter in thedorsum was created on either normal or diabetic mice, and dressed with hydrocolloid, Npole of static magnetic disks fixed to the hydrocolloid dressing with double-sided adhesivein the magnetic field group. The rats subjected to static magnetic field treatment groupwere exposed to180mT static magnetic field. On days5,12and19, four rats in eachgroup were euthanized, and the gross wound area, overall wound healing time and thehistological analysis were respectively qualified and compared. Dates of every group wereobtained through photography during the wound area measurement, and were calculatedand compared by the wound healing rate and total healing time. Histologic study of woundtissue was processed by formalin fixation and paraffin section, HE staining, the details ofeach tissue repair were observed and compared by microscope, the results show that thewound healing time of diabetic rats is longer than of normal rats, and static magnetic fieldobviously improves the skin wound healing rate and shorten the healing time in5,12, and 19days in diabetic rats. Histological analyses demonstrated that180mT static magneticfields significantly accelerated the inner repair process for the damaged diabetic tissues. Inconclusion, static magnetic field with180mT is able to remarkably accelerate the diabeticwound healing process in rats. Our findings indicate that as a kind of economic, safe andnoninvasive treatment, the static magnetic field stimulation with180mT intensity possessgreat potential to be widely used in clinics for the treatment of wound healing in diabeticpatients. |
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