Expression And Significance Of Sirt3in Human Adipose Tissues With Different Degrees Of Obesity

Objective：By testing expression level of Sirt3and LCAD gene in abdominal subcutaneousand omental adipose tissue in people with different levels of obesity (normal-weightand overweight) to explore the expression and significance of Silent informationadjustment factor3(Sirt3) in the human adipose tissue in different groups of peoplewith varied levels of obesity, as well as the relations between Sirt3and obesity. It maynot only establish a basis for the research of obesity related factors, but also lead to anew approach to the treatment of obesity in the future.Methods:1. The selection of objects of the study: patients undergoing selective abdominalsurgeries under general anesthesia. They are strictly not with malignant tumors, acuteinflammation, pregnancy, and diabetes, but with diseases such as biliary calculi,hemangioma, gastric stromal tumor, and uterine fibroids. These patients are withoutany history being treated with drugs including glucocorticoid, sex hormones, insulin,lipid-lowering drugs. There are no major changes in body weight (<10%) withinthree months in these patients, and they have never received any weight-losingtreatment or been on a diet.2. The measurement indexes: Body Mass Index (BMI), Waist Hip Ratio (WHR)and relevant biochemical index, including Fasting Plasma Glucose (FPG), BloodCholesterol (TCH), Triglycerides (TG), Low Density Lipoprotein (LDL-C), HighDensity Lipoprotein (HDL-C) and Very Low Density Lipoprotein (VLDL-c)).3. grouping of adipose tissue samples: according to BMI level and the samplingbody area, the adipose tissue samples are divided into four groups, the normal weightSubcutaneous Adipose Tissue (SAT-control) group, the overweight SubcutaneousAdipose Tissue (SAT-obesity) group, the normal weight Abdominal Adipose Tissue (VAL-control) group, and the overweight Abdominal Adipose Tissue (VAL-obesity)group.4. detecting the expression of Sirt3and LCAD among the groups: real-timePCR to detect the mRNA expression and western blot to explore the proteinexpression.Results:1. There were no significant differences (P>0.05) between the two groups ofobjects in terms of age, WHR, FPG, TCH, TG, LDL-C, HDL-C and VLDL-C.Concerning the body weight and the BMI level, the indexes of the overweight groupwere significantly higher than the normal weight group (P <0.05).2. The Sirt3mRNA expression in adipose tissue in normal-weight group wassignificantly higher than the overweight group (P <0.05).But the expression of LCADmRNA of the two groups indicated no statistical significance (P>0.05).3. In the normal weight group, the expression of both Sirt3gene and acetylatedLCAD protein are higher in the adipose tissue comparing with their overweightcounterparts. Sirt3expressions in subcutaneous fat tissues in both groups were higherthan that in abdominal fat tissue, and the differences indicated statistical significance(P <0.05). While the expression of deacetylated LCAD protein in the two groupslacked statistical significance (P>0.05).Conclusions：1. The expression of Sirt3in subcutaneous fat tissue may associate with body fatcontent. By comparison, the expression level of Sirt3in abdominal fat tissue may, to acertain extent, reflect the increase of abdominal visceral adipose tissue.2. The significantly different levels of Sirt3gene expression in separate bodyparts and people with varied obesity levels indicate its close link to energymetabolism and obesity.