The Influence Of Maternal Limb Ischemic Preconditioning On Express Change Of Apoptosis Protein Caspase In The Fetal Hippocampal Neurons Induced By Distress-reoxygenation In Rats

Object: To evaluate the expression effects of maternal limb ischemic preconditioningon fetal hippocampal neurons apoptosis protein Caspase induced by intrauterinedistress-reoxygenation in rats.Methods: The pregnant19days of Sprague Dawley（SD）rats were randomly dividedinto Sham（S）group，Control（LIP）group，Fetal distress（FD）group and the LIPplus intrauterine distress（LIP+FD）group.1）Sham（S）group:Pregnant rats were underwent laparotomy on embryonic Day19thenoff abdominal；2）Control（LIP）group: When exposed the uterus， limb ischemicpreconditioning was generated by5min occlusion followed with5min reperfusion inpregnant rats femoral artery blood flow in the right lower limb，and repeated for threecycles to establish LIP model；3）Fetal distress（FD）group: When exposed the uterus，intrauterine ischemia was induced by clamping the arteriovenous branch of the uterineand ovarian with micro artery clips for15min followed by removal of the clips topermit reperfusion to induce apoptosis in fetal rat hippocampal neurons；4）LIP plusintrauterine distress（LIP+FD）group: When exposed the uterus， intrauterine ischemiawas induced by clamping the arteriovenous branch of the uterine and ovarian withmicro artery clips for15min followed by removal of the clips to permit reperfusion. Atthe same time，limb ischemic preconditioning was generated by5min occlusionfollowed with5min reperfusion in pregnant rats femoral artery blood flow in the rightlower limb，and repeated for three cycles. Cesarean section occurred in pregnant rats (21days gestation) to aquire live pups in each group after2days.The pups were decapitated and the Caspase3，8，9positive cells in hippocampalneuronal apoptosis of fetal rat were measured by immunohistochemical stainingthrough light microscope.The expression of protein Caspase3，8，9in hippocampalneuronal apoptosis of fetal rat was detected by Western blot test and expressionchanges of protein Caspase3，8，9mRNA in hippocampal neuronal apoptosis of fetalrat was detected by the RT-PCR test.Result:1）Compared with the S group， The Caspase3，8，9positive cells in CA1hippocampus，the expression changes of protein Caspase3，8，9and the proteinCaspase3，8，9mRNA in hippocampal neuronal apoptosis of fetal rat were highersignificantly in FD group （P<0.05）.2）Compared with the S group， The Caspase3，8，9positive cells in CA1hippocampus，the expression changes of protein Caspase3，8，9and the protein Caspase3，8，9mRNA in hippocampal neuronal apoptosis of fetalrat didn’t change significantly in LIP group （P<0.05）.3）Compared with the S group，The Caspase3，8，9positive cells in CA1hippocampus，the expression changes ofprotein Caspase3，8，9and the protein Caspase3，8，9mRNA in hippocampalneuronal apoptosis of fetal rat were higher significantly in LIP+FD group （P<0.05）.4）Compared with the FD group， The Caspase3，8，9positive cells in CA1hippocampus，the expression changes of protein Caspase3，8，9and the proteinCaspase3，8，9mRNA in hippocampal neuronal apoptosis of fetal rat were lowersignificantly in LIP+FD group （P<0.05）.Conclusions:Caspase3，8，9in hippocampal neuronal apoptosis of fetal rat induced bydistress-reoxygenation in rats were downregulated significantly by maternal limbischemic preconditioning. Maternal limb ischemic preconditioning inhibited fetalhippocampal neurons apoptosis induced by intrauterine distress-reoxygenation in rats.