The Protective Effects Of Remote Ischemic Postconditioning On Cerebral Ischemia/Reperfusion Injury In Rats Which Reduces The Expression Of Chop And Caspase-3

Objective:To study the effeets of remote ischemic postconditioning (RIP) on cerebral ischemia and investgate the mechanism of postconditioning following middle cerebral artery rats.Methods:There are two part of experiment,83Sprague Dawley(SD) rats were used in the experiment. Remote ischemic postconditioning was performed by 3 cycles,of 10min bilateral femoral arteries occluded and 10min bilateral femoral arteries released. Primary part:we established the model of ischemic postconditioning and proved the protection of ischemic postconditioning for brain tissue.59 Rats were randomly divided into eight groups:(1)Sham,n=3;(2)Control,n=8;(3)Remote Postconditioning on occlusion, RIPl,n=8;(4) Remote ischemic postconditioning on reperfusion, RIP2,n=8;Rats were sacrified at 24h after reperfusion and measured infarctsize. Functional neurologieal outcome was determined at 4h and 24 h after repefusion.The second part:To investigate the protection mechanisam of remote ischemic postconditioning.24SD rats were randomized into 4 groups: (1)Sham,n=6;(2)Control,n=6;(3)Remote ischemic postconditioning on occlusion, RIP1,n=6;(4) Remote ischemic postconditioning on reperfusion RIP2,n=6.At 24h after reperfusion,the brains was obtained for chop and caspase-3 by immunohistochemistry.Results:The infarct volumes of Remote ischemic postconditioning on occlusion group and Remote ischemic postconditioning on reperfusion group, diminished compared to control group.(P<0.05) There is no diffierence between Remote ischemic postconditioning on occlusion group and Remote ischemic postconditioning on reperfusion group.(P>0.05)The functional neurological scrore is no difference between every remote ischemic postconditioning group and control group.(p>0.05) The expression of chop in cortex and striatum diminished compared to control group.(P<0.05)The expression of caspase-3 in cortex diminished compared to control group.(P<0.05)Conclusions:It is suggested the remote ischemic postconditioning of 3 cycles,of lOmin bilateral femoral arteries occluded and 10min bilateral femoral arteries released on occlusion and reperfusion offer neuoprotection.Our results imply that the neuroprotection is associated with decreasing the anti-apoptosis protein of chop in cortex and striatum and caspase-3 in cortex.