The Extraction Of Flavonoids Of Perilla Cake And The Study Of Antibacterial Properties

Perilla cake for the residue after the Perilla seed oil, but it still contains rich phenol andflavonoids, free radicals, antibacterial, anti-inflammatory, anti-oxidation, anti-tumor and so onmany kinds of biological activity and application in the field of medicine, food, cosmetics, etc.But at present, mainly used as feed, Perilla cake economic value is not high, caused the seriouswaste of resources. Residue after the experiment in Perilla seed oil （Perilla cake） as thematerial, the effects of microwave extraction, soxhlet extraction, ultrasonic assisted extractionflavonoids Perilla cake process parameters and results, as well as research extract antibacterialability, so as to basil will provide a scientific basis for comprehensive deep processing andutilization of resources. The main results were as follows:1、Choose ethanol as the solvent extraction of Perilla flavone.2、From the perspective of Perilla flavone yield consideration, the superiority of variousextraction methods are: microwave extraction> soxhlet extraction> ultrasonic assistedextraction method; Ultrasonic-in conjunction soxhlet extraction method （microwave）,extracted with soxhlet extraction method and microwave method directly Perilla flavone,compared to no advantage. Therefore, sifted Perilla seed cake, do not need through theultrasonic pretreatment, can be directly to extract the Perilla flavone.3、Microwave method to extract the best condition of Perilla flavone for: Perilla seed cake20mesh particle size, volume fraction of60%ethanol, material liquid than l:20,70min, theextraction time temperature70℃of microwave, the microwave extraction,2times the Perillaflavone extraction yield of3.008%.4、Perilla seed cake of various kinds of extract, white vinegar purple Perilla seed cakeextract （Perilla flavone concentration of2.488mg/ml） inhibit the growth of E. coli effect isbest, for the MIC of50mu L/ml of E. coli..