Research On Immunomodulatory Effects Of Polysaccharides Of Sarcodon Imbricatus (L.) P. Karst. On Mice

Sarcodon imbricatus (L.) P. Karst. is a rare wild food medicine fungi in Tibetan.Polysaccharide is one of the main active substances from Sarcodon imbricatus, which has thepharmacological activity of anti-tumor.The polysaccharide of Sarcodon imbricatus was prepared, then the immunepharmacological activity of which was investigated.The results were as follows:1. The polysaccharide from Sarcodon imbricatus was extracted by the traditional method ofwater extraction and alcohol precipitation. The lipid of the mycelium powder was firstlyremoved, and the powder was immersed at100oC for3h at the solid-liquid ratio of1:15. Theextraction was repeated three times. The extraction rate of polysaccharides was11.87%, and thepolysaccharide content was74.89%. The glucose and mannose concentrations were determinedby HPLC. The concentrations were1.55mg/mL and0.57mg/mL, and the contents were29.28%and10.83%.2. The effect of polysaccharide from Sarcodon imbricatus on normal immune function ofmice was researched. The immune organ indexes (spleen and thymus index) were determined.The monocyte-macrophage cellular phagocytosis function was measured using carbon clearancemethod and microplate reader. The T cell proliferative response levels collaborating with conAinduced by the polysaccharide in Sarcodon imbricatus was measured through culturing thenormal mouse spleen lymphocytes cell in vitro. Wound mice were treated by oral and topicalpolysaccharide in Sarcodon imbricatus, and the rate of wound healing was measured after3d,7d and11d. Wound tissue was observed after3d and7d; HE and Sirius Red Straining stainingof biopsy were determined after3d and7d. The IL-2, TNF-α and IgG contents in inducedinvasive mouse serum were measured. The results showed that each dose of polysaccharide inSarcodon imbricatus could enhance partial the immune organ index of normal mice andenhanced mouse monocyte-macrophage fine Phagocytic cells. high-dose group polysaccharidein Sarcodon imbricatus was strongly enhanced, in contrast, low-dose group was weak. Thepolysaccharide in Sarcodon imbricatus collaborating with ConA could induce lymphocyteproliferation, improve stimulation index. The effects of the most high-dose polysaccharide inSarcodon imbricatus on the group were quite obvious. Compared with the normal group, thetwo kinds of oral and topical administration were observed after3d,7d and11d. Thepolysaccharide in Sarcodon imbricatus might improve the mouse skin wound healing rate andthe levels of serum IL-2, TNF-α and IgG levels. Through the observation of thehistopathological of the wound group, the results showed that the dose of polysaccharide inflammatory reaction group was stronger than the normal group. The dose of polysaccharidesinflammatory cells reduced of the first tissue section treated for7d, increased fibroblasts scarformation and formed epidermis initially.3. The effect of polysaccharide from Sarcodon imbricatus on immune function of mice wasobserved. The immunosuppressed mouse model of immune by intraperitoneal injection ofhydrocortisone was constructed by organs weight measurement and assay of thymus index andspleen index. The carbon clearance method was used for measuring immunosuppressed micegiven monocyte-macrophage phagocytosis; injection of hydrocortisone in the miceimmunosuppressive system state, full-thickness incision with surgical scissors, making the holeeven build immunosuppressive compound animal skin wounds model. The internally andexternally polysaccharide in Sarcodon imbricatus after observing and modeling changes in bodyweight in mice generally the same. The determination of wound healing rate after3d,7d and11d was observed. HE and Sirius Red Straining staining of staining biopsy pathology andhistological changes were observed. The mouse serum IL-2, TNF-α and IgG contents weredetermined. The results showed that each dose of polysaccharide in Sarcodon imbricatus couldimprove the immune suppression of the immune organ index in mice and enhanced immunesuppression mouse monocyte-macrophage phagocytosis. After the construct of modeling, mousemuscle was weak and the coat was dull comparing with normal group. Meanwhile, the modelgroup weight reduced. Comparing with the model group, the two kinds of oral and topicaladministration group treated with polysaccharides in different dose could raise mouse skinwound healing rate and the serum IL-2, TNF-α, IgG levels were increased. Comparing with themodel group, the biopsy staining of the wound, Sarcodon imbricatus could enhance localinflammatory cell infiltration. The fibroblasts each dose group increased after7d, whichpromoted the formation of collagen fibers of connective tissue.Conclusion: Polysaccharide from Sarcodon imbricatus could be maintained or improved thecongenital immunity of the nomal mice and the immunosuppression mice caused by theinjection of hydrocortisone. Polysaccharide from Sarcodon imbricatus could also enhance theimmune function and stimulate antibody formation. Polysaccharide from Sarcodon imbricatuscould enhance the physical conditions of normal mice and induce the levels of the immunesuppression mice to be the normal levels.