| BACKGROUNDLigament injury is very common in the traffic accident, military training and sportsactivities，With the improvement of people’s living standard，ligament injury incidencewas increased yearly. Cruciate ligament injury may result in joint movement disorders，then the ligament reconstruction surgery will be inevitable. Cruciate ligamentreconstruction includs autologous ligament transplantation, allogeneic tendon graft andartificial ligament transplant. With the rapid development of materials science, theapplications of artificial ligament has made in further progress. PET has goodbiomechanical properties and has the consensus of scholars in many countries. PETcleaning and disinfection of the artificial ligament of work is the key to work safety and isrelated to material biomechanics and the stability of the spatial structure change, potentialexogenous from the material source of infection, inflammation caused by harmfulimpurities, affecting the the bone healing. The development of ideal artificial ligament,need cleaning and disinfection methods for materials, designing and improving rationalimpurity washing, disinfection treatment scheme, to meet the requirements of the bone healing.OBJECTIVEAnalyzing the different weaving structures of PET ligament of the material under theinfluence of different disinfection methods, to assess the effect of different sterilizationmethods on the material of biomechanics, discusses the feasibility and rationality ofdisinfection and impurities of washing solution, for the friendly combined with bone tissueand the implant materials provide the necessary conditions.METHODS1. Using the same line density of PET fiber as the material, weaving the pore size, thesame PET artificial ligament structure, respectively, to give ethylene oxide fumigation,high pressure steam,60Co irradiation sterilization treatment, and provided not beendisinfected blank control group, biomechanical testing, line statistical analysis of themeasurement results. Observed under scanning electron microscopy (sem) materialstructure change, Measure the pore size, porosity change.2. Chitosan graft modification of PET materials, ligament to clean steps, reagents,scanning electron microscope, cleaning effect by cell culture test, observe the cells in theearly days of material surface adhesion.3. Ligament materials implanted animal bone tunnel after12weeks, throughbiomechanical testing, hard tissue biopsies, observe the bone healing condition..RESULTS1. The high pressure steam sterilization reduced the maximum rupture strength and thefirst time rupture strength of PET material. The maximum rupture strength was reducedafter60Co irradiation sterilization. After the EO sterilization, there is no significant effecton the weave structure, the elongation at break, the maximum rupture strength or the firsttime rupture strength. The EO sterilization can be considered as a simple, safe andapplicable disinfection method for the PET microporous woven artificial ligamentmaterial.2. After the surface modification of materials, after cleaning reagent, steps, obviouslyimprove the material surface cleanliness, adhesion impurity decreased significantly, the mouse osteoblasts can grow in the material surface adhesion, growth status to normal.3. After material implanted animal bone tunnel, did not produce erosion, did not causeobvious immune rejection and inflammatory response, through the surface of the graftedchitosan molecules, material can improve biocompatibility, beneficial to the combinationof material-bone.CONCLUSION1． High pressure steam sterilization after ligament biomechanical performance ofsample decrease is more noticeable; With60Co radiation disinfection method has littleeffect on the mechanical properties of product. The method can be further improved.Ethylene oxide sterilization method can be used as a biological PET effective disinfectionmethod of artificial ligament. Weaving ligament materials using60Co, ethylene oxidesterilization method is more appropriate. The linear density, the greater the weave formingthree-dimensional microporous structure is not easily affected by the conventionaldisinfection.2． Line on the material anhydrous alcohol, alkali, surfactant, C2H2O4solution afterwashing, PET material surface impurities through full washing, ligament cells afterwashing ligament materials have the surface adhesion.3． Cleaning disinfection treatment of experimental material, the New Zealand whiterabbit implanted bone tunnel after12weeks, hard tissue slicing and mechanical test resultsshowed that new bone healing in good condition, form relatively stable material can bethought of bone healing.In conclusion, the polyethylene terephthalate material, through the test after themodification, cleaning and disinfection, can be thought to have better biocompatibility andsafety. |
PDF Full Text Request