Study On The Structure And Activity Relationship Of Polysaccharides And Steroids From Inonotus Obliquus

In order to illustrate the structure and activity relationship of polysaccharides and steroidsfrom Inonotus obliquus, the sclerotia of Inonotus obliquus were extracted to give P. E.-solublefraction (PEF), EtOAc-soluble fraction (EAF), n-BuOH soluble fraction (n-BF) and H2O-solublefraction (WF) four fractions. Chromatography methods were used to isolate and purify PEF andEAF to obtain nine compounds. Six of these compounds were identified as lanosterol (1),ergosterol (2), inotodiol (3), ergosterol peroxide (4), trametenolic acid (5) and3-acetyloxylanosta-8,24-diene-21-ol (6) respectively.Their anti-inflammatory ability were determined on the mice macrophage RAW264.7cell,with the purpose of finding the active anticancer compounds and illustrating the relationship ofanti-inflammatory and structure. The anticancer activity of ethanol extraction, four fractions andsix compounds were evaluated on the cancer cell in vitro, which aims at finding the activeanticancer compounds and illustrate the relationship of anticancer and structure. Results indicatedthat PEF and EAF showed a good inhibitory effect on PC3and MDA-MB-231. For sixcompounds, all exhibited inhibition properties on cancer cells except of lanosterol (1) andinotodiol (3). All extraction, fractions and compounds were found to significantly inhibited NOproduction; significantly inhibited NF-κB production except of n-BF, WF, lanosterol (1),ergosterol (2) and inotodiol (3).Besides, DEAE anion exchange chromatography was used to purify IOPS and constituenteluted with0.1M NaCl (IOPS-2) solutions was selected as the subject of this study. IOPS-2wasmodified with different methods. The changes of morphological characterization, conformation,molecular weight distribution and monosaccharide constituents were characterized. Theantioxidant activities in vitro of IOPS-2and its derivatives were determined in order to understandtheir SAR. Results illustrated that modification to IOPS-2led to the changes in morphologicalcharacterization, conformation, molecular weight and monosaccharide constituents; theantioxidant activities of derivatives by hot, ultrasonic and acetylation was enhanced.In summary, steroids were responsible for part of the anticancer and anti-inflammatoryeffects.The enhanced antioxidant abilities of derivatives may be due to the changes inmorphological characterization, conformation, molecular weight and monosaccharide constituents.