| Aim: To investigate the effects of Inonotus obliquus polysaccharides on the immunefunction of normal mice. Discuss the influence on the immunocyte made by Inonotusobliquus polysaccharide. Methods : Extracted by water, concentrated, deposited byethanol, get rid of albumen, dried in vacuum, after those procedures the Inonotusobliquus have been Inonotus obliquus polysaccharides. Implement lavage on healthymice using different doses of Inonotus obliquus for two weeks with polysaccharidesbrine in physiology as antitheses. Through the hemolytic trial you can realize theeffects on the humoral immune function in the healthy mice made by Inonotusobliquus polysaccharide, thenidentify the best effective dose. Use this dose ofInonotus obliquus polysaccharides in lavage experiment on mice for two weeks.Enregister the course of macrophage phagocytosis test against chicken erythrocytesand the transformation of lymphocytes in the experiment, understand the wholesystem of monocyte-macrophage phagocytosis and cell transformation oflymphocytes.Results : In the hemolytic plaque test, different doses of Inonotus obliquuspolysaccharides 50mg/kg, 100mg/kg, 200mg/kg, 400mg/kg, 800mg/kg dose group.compared with the antithetical group. The humoral immune function can besignificantly improved in the 200mg/kg group (P＜0.01); There was an increase inother dose groups compared with the antithetical group but not significantly different.using 200mg/kg of Inonotus obliquus polysaccharide as the best dose group, throughthe peritoneal macrophage phagocytosis and lymphocyte transformation test withbrine as a antithetical group. 200mg/kg of Inonotus obliquus polysaccharide can makea significant difference (P＜0.05). Conclusion : (1) Inonotus obliquus polysaccharide group can significantly improve the humoral immunity function, the immune functionand peritoneal macrophage phagocytosis of mice with the brine antithetical group. |
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