| Objective:Study on the Quality of Aquilaria agallocha by "Quintana made incense technology".Aquilaria sinensis plant resources diversity studies for the effective use of it resourcesprovide the basis.Methods:(1) By collection and arrangement the ancient herbal books about the description ofthe Aquilaria sinensis, from it’s medicinal history, the evolution of species, different namediscrimination, origin changes and edgeworthia methods.(2) Results of the new method to evaluate the quality of medicinal Aquilariaagallocha, according to the2010edition of "Chinese Pharmacopoeia"(a) under Aquilariaagallocha content and increased fluorescence microscopic identification, ether-solubleextract content determination, while using different extraction methods Aquilaria agallochaherbs volatile components for GC-MS analysis, and the use of gas chromatography inAquilaria agallocha herbs characteristic active ingredient benzyl acetone content.(3) To research the Aquilaria sinensis plant leaves, fruits, seeds and xylem morphology,analysis the phenotypic diversity of Aquilaria sinensis plant, and through cluster analysis toclassify the Aquilaria sinensis plant resources.Results:In ancient herbal medicine records, Aquilaria agallocha plant sources includingThymelaeaceae plant Aquilaria agallocha (imports) and Aquilaria sinensis (domestic) twodifferent varieties, many different names and product specifications, and some because of origin varies, some due for varies in different parts of plants. MI Xiang always products iniJiao Zhou (in some areas of Guangxi and Vietnam) and Aquilaria agllocha to JiaoZhou for the title, it can also be said for the import of MI Xiang as Aquilaria agallocha;Qinan (chess Nan, Nan Qi, Qi blue, Qi Nan), Jianan that is the Aquilaria agallocha of thegood person, and mainly refers to the origin of the Zhancheng (now southern of Vietnam)and other countries ’s Aquilaria agallocha, namely import Aquilaria agallocha, and laterproduced in Hainan, the quality of the soil Aquilaria agallocha also known as Jianan;daughter of incense that Wan Xiang, and Wan Xiang as Aquilaria agallocha; chicken Hong,horseshoe incense, incense Tau is because of its shape and the name; ripening root ofincense is takenas Huang Shuxiang; green Guixiang twigs compaction is not bad for theperson; Zhan Xiang as part of the trunk proceeds; Jian Xiang is frying as semi-submergedin water, incense, and in terms of quality is divided into three classes: those for the previoussubmerged in water (Aquilaria agallocha), semi-submerged floating half those times (stackincense, fry), floats were most followed (Huang Shu incense).In origin, the ancient China has been documented two categories of domestic andimported origin, and with the passage of time, place names have become more specific,mainly produced in Guangxi, Guangdong (early and more in western Guangdong,Guangdong Ming Dynasty transplant in the eastern region), Hainan (in white wood-based)and Vietnam and other Southeast Asian countries (mainly in incense), and the eastern partof Guangdong Dongguan area although in the Ming Dynasty was transplanted here, butrather more famous geological suitability.Herbal incense described in the main junction comprising two kinds of natural factorsand human factors, natural factors have cooked knot, shedding and insects, human factorsprompting knot incense animate knot that knifed, branding iron flashes of red and "opendoor incense."(2) Five batches of new methods knot incense Characters are: irregular oblong pieces,thickness0.1~0.7cm, surface (slightly) rough, with marks; light tan to dark brown with asmall amount of white wood resin blocks alternating with there droplets pitting one sideand sallow deadwood like; quality than the hard, dry and brittle, loose inside, the air is fragrant, spicy taste, chewing Ma tongue.Microscopic observation of the powder are: fiber tracheids more bundles, thickerwalls, wood, edge and more straight, pits more, ends abruptly tapering; tough fibers longspindle cell wall wavy; catheter catheter is bordered pits, closely arranged bordered pits,mutual columns, intraductal yellow brown resin clumps, often broken prolapse. Observedunder a fluorescence microscope: Powder significant gray-green fluorescence.TLC sample and the reference drug in the same position in the same color spots, andalcohol-soluble extract content of13%to21%, much higher than the10%ofPharmacopoeia, ether extract content of1.6%~9.8%between.After ethanol extract micro-sublimation, was yellowish brown oil, aroma; theirreaction to light cherry red color, the color is slightly deepened after placement.Volatile components by GC-MS analysis identified a total of151components, ofwhich94reported ingredients are predecessors did. And151components sesquiterpene63,aromatic40,37as well as other types of ketones with a color10fatty acids, and naturalincense to sesquiterpenes, aromatic, fatty acids, color ketone-based components of similarcomposition and characteristic detected active ingredient incense incense coil alcohol,benzyl acetone.All samples in the content of benzyl acetone in0.03~2.5mg/g.(3) Different Aquilaria sinensis greater differences between plant resources, and thedifferent traits between different samples also showed varying degrees of diversity. If thetip of morphological variation among the largest coefficient for44.08%; followed by thekind of hook or without spikes, seed sharp hairs, leaf width, respectively,34.90%,30.03%and24.00%; leaf length, fruit length, stalk length, fruit length to diameter ratio ofcoefficient of variation of10%or more; tip shape, leaf base shape, blade aspect ratio, fiberlength, fiber diameter, fiber length to diameter ratio, a long catheter, the catheter diameter,length and diameter of the catheter ratio, arrangement of xylem vessels are on the1%.Through cluster analysis methods can not be Aquilaria sinensis plant leaf morphologybased on the size of the resource is divided into three categories, and the arrangement ofxylem and leaves, fruits, seeds endures no necessary connection between the form and can not be judged through the external form xylem arrangement of the catheter.Conclusion:(1) Ancient herbal Aquilaria agallocha plant sources described includeThymelaeaceae plant Aquilaria agallocha (imports) and Aquilaria sinensis (domestic) twodifferent varieties. MI Xiang, Jianan, Qinan, Wan Xiang, daughter Aquilaria agllocha_aresynonyms, yellow wax Shen, chicken incense, Zhan Xiang, Qing Guixiang, horseshoeincense, Tau, fried incense, incense and other Huang Shu for Aquilaria agllocha differentcommercial specifications. Mainly produced in Guangxi, Guangdong, Hainan (in Aquilariasinensis-based) and Vietnam and other Southeast Asian countries (mainly in Aquilariaagallocha), mostly in western Guangdong early Ming Dynasty when transplanted in theeastern part of Guangdong. In the end method described on the main natural factors(cooked knot, shedding and insects) and human factors (raw knot that knifed, branding ironflashes of red and "On_Gate") two categories.(2) Aquilaria sinensis "henselae made technology," the knot Aquilariaagallocha addition traits, but in microscopic, physical and chemical aspects of the researchresults are in line with the requirements of the Chinese Pharmacopoeia, volatilecomponents analysis151components were identified, of which94components were firstreported, and detecting a characteristic of the active ingredient Aquilariaagallocha Aquilaria agallocha coil alcohol, methyl chromone, benzyl acetone; like allnatural Aquilaria agallocha to sesquiterpene, aromatic, fatty acids, ketones, mainlycomposed of color, so that the new method the knot with natural Aquilariaagallocha Aquilaria agallocha similar, can replace natural Aquilaria agallocha medicine.(3) Aquilaria sinensis more complex diversity of plant resources, according to the leafmorphology of plant resources can not be divided into three categories, and thearrangement of xylem and leaves, fruits, seeds between extrinsic forms are not necessarilylinked. |
PDF Full Text Request