Effect Of Lycium Barbarum Polsaccharides(lbp) On The Expression Of Lectin-Like Oxidized LDL Receptor-1in Rat Vascular Smooth Muscle Cell Induced By High Glucose

Objective To observe the impact of the high concentration of glucose on theexpression of LOX-1gene on rat vascular smooth muscle cell lines (A7r5)in vitro, as well asthe effect of LBP on the expression of LOX-1on the high glucose-induced cells.MethodsThe inverted microscope observation of cell growth, using RT-PCR, Western blot analysis todetect cells were incubated with different concentrations of D-glucose (5mM,10mM,15mM,20mM,25mM,30mM,40mM) for48h, and cells were incubated with glucoseconcentration25mM for different time (0h,24h,48h,72h), the expression of LOX-1mRNA and protein in A7r5cell; glucose concentration25mM with different concentrations(60μg/ml,80μg/ml,100μg/ml) of LBP co-cultured cells48h and the cells were incubatedby the LBP concentration of100μg/ml and glucose25mM for different time (0h,24h,48h,72h),the expression of LOX-1mRNA and protein in A7r5cells. As the internal standard ofβ-actin primers. Each repeated three times.Result1. With the glucose concentrationincrease and the expression LOX-1mRNA and protein also gradually increased, while theglucose concentration of40mM, the expression of LOX-1mRNA and protein reached thepeak (P <0.01); different incubated times of the same concentration of glucose, LOX-1mRNA and protein expression increased with time, reached a peak for48h (P <0.01), andthen decreased.2.Different concentrations of LBP can inhibit the expression of LOX-1mRNA and protein, the peak of the strongest inhibition is the concentration of100μg/ml; thesame concentration of LBP at different times the expression of LOX-1mRNA and proteincan be reduced with time, and72h can reached the peak.(P <0.01). Conclusions1. High concentrations of D-glucose can improve the expression of LOX-1mRNA and protein,andthis phenomenon is depend on concentration and time.2. The expression of LOX-1mRNAand protein can be inhibited by LBP and the higher concentration of LBP inhibitory effectis more obvious.