| Objective To study the associated of CC subfamily of chemokines macrophageinflammatory protein-1α (macrophage inflammatory of protein1-alpha, MIP-1α, CCL-3) atthe clinical level and disease is more and more attentioned,Also,to study the genetic levelMIP-1α and the prevention of disease, occurrence, development and prognosis is more andmore be concerned to many medical scientists.MIP-1α, macrophage, monocyte chemotacticactivity and can induce inflammatory cytokines, which can influence the occurrence anddevelopment of the granuloma. The relationship of MIP-1α gene single nucleotidepolymorphism (SNP) and the disease also gradually been recognized. This issue through theMIP-1α gene rs1130371(C/T), rs1719134(A/G) two polymorphisms (SNPs) associated TBresearch is needed to explore its association with tuberculosis genetic susceptibility.Methods case-control study were used in this research uses to collect Ningxia Haiyuanarea154patients were clinical diagnosed of tuberculosis as cases, the mean age at(43.41±19.65),The gender ratio is79:75, Ningxia Haiyuan area190healthy people were selected whosesex and age matched as control group, the mean age at(43.11±15.90),The gender ratio is91:99;The polymerase chain reaction-restriction fragment length polymorphism analysis(PCR-RFLP)was used to genotyp rs1719134(A/G). At the same time each100of case-control samples ofNingxia area were collected Randomly. the average age of respectively case (43.36±22.36),control(42.35±21.97).The sequencing method was used to genotype rs1130371(C/T), SPSS11.5statistics software was used to calculate the genotype, allele frequency, and chi-square test, atthe same time use the SHE sis software to2SNP loci by linkage disequilibrium analysis.Results1The MIP-1α gene rs1719134locus and tuberculosis genetic susceptibility analysis results:1.1Haiyuan sample rs1719134genotype distribution: in the case group the AA genotypewas27.9%,the AG genotype was63.0%, the GG genotype was9.1%, in the control group theAA genotype was17.9%, the AG genotype was71.1%,the GG genotype was11.1%. Chi-squaretest was used with SPSS software, the results show that the AA genotype distribution of bothcase and control groups were significantly different (P=0.027,OR=1.777(1.066-2.964)).2The MIP-1α gene rs1130371locus and genetic susceptibility to tuberculosisanalysis:each of100cases and control group were randomly selected, these samples weresequenced, the results showed that the CC, CT genotypes and allele C and T in case-controlsamples no significant differencein the case-control sample the distribution was no significantdifference(P>0.05). In this study, found that the total sample of200cases there was no TTgenotype.3SHE sis software was used to analysis the MIP-1α gene rs1130371and rs1719134linkage disequilibrium, and D ’value of0.209and r2value of0.003, indicating the linkagedisequilibrium association of these two SNPs not closely.4Four pairs of MIP-1α gene rs1130371, rs1719134and the RANTES gene IN1.1points,403points,28points each genotype SHE sis software linkage disequilibrium D ’values <0.8,indicating the linkage disequilibrium association between MIP-1α and RANTES gene SNPsnot closely.Conclusion1The MIP-1α gene rs1719134(A/G), and the incidence of TB in the the Haiyuanindependent samples associated wild-type AA genotype,may be a predisposing factors to TB.2The MIP-1α gene rs1130371(C/T) in the Ningxia population sample of200cases ofCC, CT from two genotypes did not find the TT genotype.Of CC, CT genotype distribution and Ningxia population TB no apparent connection. |
PDF Full Text Request