Comparative Analysis Of Synovia Proteomes And Serum Of Demineralized Osteoarticular Allograft Transplantation In Rabbits

Objective:Many scholars at home and abroad are engaged in the study of articular cartilageinjury for a long time. A large number of experiment has confirmed that the growthfactor plays an important role in maintaining the normal form of articular cartilage.There are also many researchs show that the allosome demineralization joint playsinduction in the process of articular cartilage repair damage. This experiment isimplanted the allosome demineralization joint into the right defect elbow joint of NewZealand big rabbits. Using body fluids proteomics method to analyze the samples ofserum and synovia whether have the same protein after transplantation,which aredifferent from the control groups. The experiment in order to find specific proteinsduring the articular cartilage regeneration process which presented in body aftertransplantation.And use it to study the related proteins’ function mechanism andmodel in the articular cartilage regeneration process and look for promoting the keylink of articular cartilage regeneration to provide new ideas and new clues.Methods:A total number of16adult New Zealand white rabbits were divided randomly into3groups, ie. groupⅠa twenty-millimeter osteoarticul defect with the perinsteum wascreated in the proximal part of radius in8New Zealand white rabbits,which weretransplanted with demineralized osteoarticular allografts. As controls, group Ⅱatwenty-millimeter osteoarticul defect with the perinsteum was created in the proximalpart of radius in4New Zealand white rabbits,which were left alone. groupⅢ the normal New Zealand white rabbits,which contains4. First of all to establish thepositive control group and produced the demineralized osteoarticular allografts, thento establish the experimental group. Since then we respectively collected ones of3groups synovia samples and serum ones in4weeks,8weeks,12weeks,16weeks andput them into2D-PAGE and MADLI-TOF-MS to analyse,then turned to the relateddatabase to identify the result.Results:The New Zealand big white rabbits’ elbow articulation chondral defect modelswere built successfully, and transplanted the homemade demineralizationosteoarticular allograft to trigger the body to repair the elbow articulation cartilagedefects. And then through the two-dimensional gel electrophoresis to separate the variousproteins,which were produced from the articular synovial fluid and serum, found the joint fluidgroup、 positive group compared with blank group respectively, the experimental serumgroup、 positive group compared with blank group respectively, there were significantdifferences protein spots. According to the experiment principle,we found30significative differential protein spots in gel-analysis as compared with controls. Thefurther MADLI-TOF-TOF-MS analysis revealed three peculiar kinds ofproteins,which present in the experimental serum,whose GenInfo identifier numberwere136466、126722803、 and126723746,those might be associated to theregeneration of elbow articular cartilage defects.Conclusion:In the process of articular cartilage regeneration, serum produced three specifickinds of protein, which may play important roles in the right elbow articular cartilageregeneration. One kind of TTR protein whose GenInfo identifier number was136466,which belongs to the transthyretin family and may through the neuroendocrine systeminvolved in genetic replication, repair and expression. Another kind of ALB proteinwith the GenInfo identifier number126722803, which is the main protein of plasmaand has important functions,such as with good ability of combination, oxidationresistance, reduce the cell toxicity, protect cells from damage and oxidation etc. The other kind of RBP4protein with the GenInfo identifier number126723746whichbelongs to the calycin superfamily. Lipocalin family and interaction with thyroidelement, through the neuroendocrine system involved in starting the process of geneexpression. This finding may further reveal the nature of articular cartilageregeneration process and for a more in-depth study of articular cartilage regenerationmechanism lay the foundation.