The Role Of URATv1in Development Of Uric Acid Stone

Uric acid stones are more common types of urolithiasis. The literature shows thatapproximately15%uric acid stones of all calculis in China and abroad reported that5%to39.5%. Due to the high rate of recurrence of uric acid stones, the understandingof the pathogenesis of uric acid stones, the effective prevention and treatment of uricacid stones have important clinical and social significance. General research point ofview that uric acid stone development associated with high uric acid in urine disease.Uric acid of original urine reabsorption to reduce the lead to the accumulation of uricacid in the original urine, thereby causing an increase in uric acid concentrations.URATv1, organic ion transporters in human renal proximal tubular brush border, ismainly responsible for the uric acid transporter in renal tubular epithelial cell, andoccupies an important position among the reabsorption of uric acid. Occur when itsfunction abnormalities may lead to reduction in uric acid reabsorption, increasedlevels of uric acid in the original urine. The results show that the addition to encodingURATv1protein SLC2A9gene can lead to high uric acid in urine disease struck inthe dog, SLC2A9urinary stone formation, but no studies have reported humanURATv1results for the statistical analysis of clinical specimens, the URATv1 dysfunction on the ability of uric acid transporter, and polyuria with high uric acid.Object To study the absorbility of uric acid after the the expression ofURATv1was inhibited in HK-2cells.Materials and methods siRNA Plasmids with green fluorescent protein weretransfected into HK-2cells to inhibit the expression of URATv1.24hours aftertransfection, expression of green fluorescence proteins were observed withfluorescence microscopy, and the expression of URATv1in protein and mRNA levelwere measured by western blot and real time fluorescence quantity PCR in HK-2cellsrespectively. Finally the absorbility of uric acid was checked by using uric acid kit.Results Green fluorescent protein was discovered after transfection for24h inHK-2cells.. The expression of URATv1was decreased both in protein and mRNAlevel. Consequently, the absorbility of uric acid of HK-2cells was reduced.Conclusion The absorbility of uric acid was decreased when the expressionof URATv1was inhibited in HK-2cells, which may result in hyperuricuria, andresponsible for the formation of uric acid stone.