Function And Regulation Of 3-Methyladenine DNA Glycosylase In Mycobacterium Tuberculosis

Mycobacterium tuberculosis (M tuberculosis) is a highly infectious pathogen, which can cause lung tuberculosis (TB) when infect humans and animals. M. tuberculosis can persist for a long time in host cells because of its strong ability in gene mutation and drug resistance. However, the mechanism of mutations and DNA repair in M. tuberculosis remain unclear. In this study, we have investigated the function and regulatory mechanisms of 3-Methyladenine DNA Glycosylase (Rv1688) involved in DNA repair in M. tuberculosis. The main results obtained are as follows:(1) Using bacterial two-hybrid technique, we identified a number of proteins interacted with Rv1688, including a TetR type transcription factor Rv0825c. (2) Rv0825c was found to improve the DNA-binding activity of Rv1688 and thus stimulate its DNA cleavage activity. (3) The interaction between two homologous proteins in Mycobacterium smegmatis has been shown to be conserved. (4) Rv0825c can interact with the promoter sequence of Rv1688, and, therefore, it may also regulate the expression of Rv1688 gene.There is no research on its 3-Methyladenine DNA Glycosylase in the important human pathogen M. tuberculosis. Its function and regulating mechanisms remain largely unclear. However, this type gene may play a key role in DNA excision repair and it has a direct impact on mutation and drug resistance of pathogen. Our study on the function and regulatory mechanism about the enzyme of M. tuberculosis will help us understand the drug resistance of TB in depth. Obviously, a further study remains to be done.