Screening The Bacteriostatic Active Composition And Toxicological Preliminary Estimate On Traditional Korean Medicine Of Rhizoma Aetractycodes Japonlca

Objective: To study the antibacterial activity of extracts from Rhizoma Atractylodis Japonicae (A.J) which is a traditional Korean medicine. Observe the acute toxicity of A.J powder, super micro powder and the water extract of it. For rational utilization of medicine resources, and to promote the development of safe, reliable and low toxic anti-bacterial preparation and related products. Method:1. Through the bacteriostatic effect on Streptococcus mutans (KCTC3065), the bacteriostatic effective part was selected by measuring the minimum inhibitory concentration (MIC). The chemical compositions and contents of effective part of A.J were analyzed by GC-MS, then using silica gel column, Sephadex LH-20, octadecyl silica gel chromatography and high performance liquid chromatography combined with bioactivity-guided method, the bacteriostatic active compositions were isolated and preliminarily identified.2. Median lethal dose (LD50) of A.J powder and super micro powder in mice was determined by modified Kaber method; the water extract of A.J were administered with the maximum tolerated dosage (MTD).Results:1.Under this screening system, the A.J was extracted by using CO2 supercritical extraction (SFE) showed stronger antifungal activities against Streptococcus mutans, the minimum inhibitory concentration of it was 25μg·mL-1. Fraction was researched after separation by column chromatography and bioactivity-guided method, the structure of the compound was elucidated on the basis of physicochemical and spectroscopic methods, results showed that the bacteriostatic active was Ditridecyl phthalate. To the best of our knowledge, this compound is the first isolation from Atractylodes DC. Species.2. Median lethal dose of A.J powder was 24.91 g/kg,95% confidence interval was 22.47-27.61 g/kg;median lethal dose of A.J super micro powder was 28.53 g/kg,95% confidence interval was 25.72～31.64 g/kg;the maximum administration dosage was 495 g of A.J, the extract of A.J given to mice per kilogram, equivalent to the men with 60 kilogram whose daily administration dosage(10～30g) by 990～2970 times. Conclusion: A.J showed stronger antifungal activities against Streptococcus mutans, it can be used as a research material of natural plant bacteriostatic agent; the gastric perfusion of the extract of A.J was considered as a substance with no toxicity in this study.