| A water-soluble sulfated glucan, SHG, was isolated from Hedysarum polybotrys Hand.-Mazz by hot water extraction, anion-exchange on DEAE-52 cellulose and gel-permeation chromatography on Sephadex G-200. Elemental analysis indicated that SHG was a sulfated polysaccharide containing small amount of sulfate groups (1.47% in mass). The molecular weight was estimated to be 1.72×105 Da by high-performance gel permeation chromatography (HPGPC) and size exclusion chromatography-multi angle laser light scattering (SEC-MALLS). SHG took random coil compact conformation in 0.1 M NaNO3. Compositional analysis revealed that SHG was composed of glucose only. On the base of partial acid hydrolysis, methylation analysis, gas chromatography-mass spectrometry (GC-MS), Fourier transform (FT-IR) infrared spectra, nuclear magnetic resonance (NMR) spectroscopy of 1H,13C,β-correlation spectroscopy (β-COSY), total correlation spectroscopy (TOCSY), heteronuclear single quantum coherence (HSQC), and heteronuclear multiple bond correlation (HMBC), the results showed that SHG was composed ofα-D-(1â†'4)-linked glucopyranosyl residues, with branches at C-6 consisting of non-reducing terminal approximately every eight residues, along the main chain. Sulfate groups may attach to the backbone at O-6, which one sulfate esters occasionally occur per 38 anhydrous glucose units.We investigate the anti-radiation effect of Hedysarum polybotrys polysaccharides (HPS) on Kunming male mice. The results indicated that 40%HPS (100mg/kg),30%HPS, and 70%HPS could raise the survival rates by 33.33%,26.66%,26.66%, and extend average survival time remarkably.40%HPS (100mg/kg),30%HPS, and 70%HPS could increase WBC and lymphocytes and release the irradiation injury from X-rays.40%HPS didn’t exit a quantitiy-effect relationship. However,50%HPS could not raise survival rates and had no anti-radiation effect. |
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