Study Of Differentiating Agaricus Blazei Murill、Grifola Frondosa And Volvarietlla Volvacea Germplasm Resources By Using Molecular Marker

The problem that diversity in phenomenon with the same name and different variety or the same variety coming from different site with different name troubled the study and the production of edible fungi.Three molecular markers,namely RAPD, SRAP,ISSR,were used in the study the genetic diversities and relationships of 27 cultivated Agaricus blazei Murrill strains,74 cultivated Volvariella Volvacea strains and 30 cultivated Grifola frondosa strains 46 SCAR markers was also established according to polymophic bands.134 polymophic bands were obtained in study of 27 cultivated Agaricus blazei Murrill strains.According to the cluster analysis,27 Agaricus blazei Murrill cultivated strains were clustered into 9groups in the 70.00%dissimilarity level.10 SCAR markers were also established 27 Agaricus blazei Murrill strains were classified into 10 classes by 10 SCAR markers.82 polymophic bands were obtained in study of 74 cultivated Volvariella Volvacea strains.According to the cluster analysis,74 Volvariella Volvacea strains were clustered into 7 groups in the 26.83%dissimilarity level.27 SCAR markers were also established 74 Volvariella Volvacea strains were classified into 44 classes by 27 SCAR markers.77 polymophic bands were obtained in study of 30 cultivated Grifola frondosa strains.According to the cluster analysis,30 Grifola frondosa strains were clustered into 10 groups in the 77.41%dissimilarity level.8 SCAR markers were also established.30 Grifola frondosa strains were classified into 10 classes by 8 SCAR markers.