Physiological Mechanism Of Potassium Nutrition On Quality Formation Of Angelica Sinensis

Objective:Angelica is a daodi herb of Gansu Province.Itis known as“Ten Prescript Part Nine”andis commonly used in clinical medicine.Recent years, the imblance nutrient and decreased nutrient utilization rate lead to the quality decrease along with the eapansion of cultivated areaof Angelica sinensis, and threat to quality of crude drugs, pieces, preparation and the international reputation.K+ is an activator of more than 60 kinds of synthases, oxidases and transferases in plants. It promotes photosynthesis though effect the photosynthesis capacity of plant. It also participate the carbohydrate synthesis, transportation andtransformation, which is in favor of the form of ATP and organic acids, and further regulation the carbon and nitrogen metabolism of plant, ultimately improve the yield and quality of crops. However,there is rarely reported about the mechanism of potassium nutrition on the quality formation ofA.sinensis. The workdeep into study physiological mechanism of potassium on quality of A.sinensisin daodi regions with the help of plant physiology and ecology, plant nutrition, crop culture, Chinese medicine chemistry, Chinese medicine identification and plant molecular biology and other scientific theories and technical means, aimed at seeking the demandrule and utilization ratio of potassium elementto achieve high quality and high yield A.sinensis. Meanwhile, reveal the physiological essences about the quality inform of A.sinensis, andprovide scientific theoretical basis forexploring the nutritional regulation pathway, keeping the yield and improving the quality of medicinal herbs.Methods:Through on the spot investigation, marking, sampling, the influence factors of Angelica plant morphology were observed and determined in accordance with the 《Chinese Pharmacopoeia》(2015 Edition) recorded method of water samples of Angelica extract, content determination; Determination of the photosynthetic fluorescence characteristics measurement system and 6400-2 * 3 LED red and blue light leaves the living room using the LI-6400/XT portable photosynthesis; Determination of content of soluble protein by Coomassie brilliant blue G-250 method; Determination of glucose by UV colorimetry, fructose, sucrose, sucrose synthase(SS), sucrose phosphate synthase(SPS), pyruvate kinase(PK) and malate dehydrogenase(MDH) content; Determination of glutamine synthetase by colorimetric method(GS), glutamate synthase(GOGAT) and total amino acid content; spectrophotometry determination method of nitrate reductase(NR) activity; Determination of allantoin by enzyme activity using the standard meter method; To establish a high performance liquid chromatography(HPLC) method was developed for the simultaneous determination of ferulic acid and ligustilide, European Angelica lactone A, senkyunolide I, butylidene phthalide, senkyunolide A, senkyunolide H, ferulic acid loose sandarach eight categories of chemical composition determination method, and under the different fertilization methods, different K nutrition level of the eight components were determined.Results:(1) For the two different ways of fertilization, K300 group was better to improve the plant height、root diameter, stems and leaves fresh weight, root fresh weight, root length, body dry weight、body coarse、head dry weight、biomassetc., andpromoted the growth andimproved the yield of A.sinensis,.The second was the K150 group.(2) For the two different ways of fertilization, the K300 group better promoted the formation of Parenchyma cells, starch granule, conduit and oil chamber, andimproved the yield and quality of A.sinensis.(3) For the two different ways of fertilization, the K300 group better promoted the leaf photosynthetic rate of A.sinensis, stomatal conductance, transpiration rate, apparent CO2 use efficiency and light open PSII reaction center excitation energy capture efficiency, the PSII reaction center charge separation the actual quantum efficiency, photochemical electron transfer of share, PSII electron transport activity and photochemical pathways of excited can account for access to PSII total excitation energy ratio, dark reaction of CO2 assimilation rate, and promoted the light energyabsorption and organic matter accumulation, and improved the yield and quality ofA.sinensis.(4) For the two different ways of fertilization, group K300 better promotedthe accumulation offructose, sucrose, total amino acid content in the leaves of A.sinensisand the activityof SPS, PK, SS, MDH, GOGAT, GSH, GSH, POD, CAT, SOD, allantoinase etc., and, inhibited the activity ofGS, NR, the enzymatic phospholipase D and the content of shedding acid and total soluble protein in the leaves of A.sinensis.(5) For the two different ways of fertilization,group K300 better promoted the content of ferulic acid、ferulic acid loose sandarach、ligustilide、European Angelica lactone A、senkyunolide, and enhance the quality of A.sinensis.(6) The correlation analysis showed that there had a difference relationship among ehight chemical composition and different biochemical indexes which illustratedthat the quality of A.sinensis is affected by a variety of physiological and biochemical indicators.