| In this paper, Japanese sea bass(Lateolabrax japonicus) and white shrimp(Litopenaeus vannamei) larvae were as the object to investigate the effects of different protein hydrolyzates on growth performance, feed utilization, body composition and non-specific immunity. Cage culture experiments was taked in the L. japonicus experiment, while running water culture was used in the L. vannamei experiment. The results and conclusion are summarized as follows:1. This experiment was conducted to investigate the effect of four protein hydrolysates for replacing fish meal protein of basal diets on growth performance and non-specific immunity of Japanese sea bass(Lateolabrax japonicus). The control(FM) group included fish meal as the single protein source. The experimental groups were replaced 10% of fish meal protein with four protein hydrolysates, including fish protein hydrolysate(FPH), pig blood protein hydrolysate(PBPH), Soybean protein hydrolysate(SPH) and yeast protein hydrolysate(YPH). Five diets were formulated to be isonitrogenous and isolipidic. All diets were fed in triplicate groups of L. japonicus(initial weight 31.99 ± 0.05 g) for 8 weeks. Results indicated that the final body weight and specific growth rate(SGR) were significantly improved when fish were fed the FM and FPH groups compared with the PBPH, SPH, and YPH groups(P < 0.05), but there were no significant impact on survival rate and feed intake among all groups(P > 0.05). There were no difference in feed efficiency ratio(FER) among the FPH, FM, PBPH, and YPH groups(P > 0.05), while FPH group was significantly higher than the SPH group(P < 0.05). The FPH group had significantly higher protein efficiency ratio(PER) than the other dietary treatments(P < 0.05). The FPH, FM and YPH groups shown significantly higher protein productive value(PPV) compared with that of PBPH and SPH groups(P < 0.05), while PPV in the FPH, FM and YPH groups was not significantly different(P > 0.05). AKP, ACP, SOD, LZM activities and T-AOC in liver and serum of the FPH and YPH groups were significantly higher than other groups(P < 0.05). In conclusion, FPH had the best increasing tendency on growth and non-specific immunity compared with other diets, followed by YPH, PBPH and SPH.2. This study was conducted to investigate the effect of four protein hydrolysates for replacing fish meal protein of basal diets on growth performance and non-specific immunity of Pacific white shrimp(Litopenaeus vannamei). The control(FM) group included fish meal as the single protein source. The experimental groups were replaced 10% of fish meal protein with four protein hydrolysates, including fish protein hydrolysate(FPH), pig blood protein hydrolysate(PBPH), Soybean protein hydrolysate(SPH) and yeast protein hydrolysate(YPH). Five diets were formulated to be isonitrogenous and isoenergetic. All diets were fed in triplicate groups of L. vannamei(initial weight 2.39 ± 0.10 g) for 8 weeks. Results indicated that the final body weight and specific growth rate(SGR) were significantly improved when fish were fed the FM and FPH groups compared with the PBPH, SPH, and YPH groups(P < 0.05), but there were no significant impact on survival rate among all groups(P > 0.05). The FPH and FM groups shown significantly lower feed intake compared with that of PBPH and SPH groups(P < 0.05), while feed intake in the FPH, FM and YPH groups was not significantly different(P > 0.05). There were no difference in feed efficiency ratio(FER) and protein efficiency ratio(PER) among the FPH, FM, and YPH groups(P > 0.05), and FPH and FM groups was significantly higher than the PBPH and SPH groups(P < 0.05). The FPH, FM groups shown significantly higher protein productive value(PPV) compared with that of SPH groups(P < 0.05), while PPV in the FPH, FM and YPH groups was not significantly different(P > 0.05). There were no difference in AKP, ACP, NOS activities and T-AOC of serum among FPH and YPH groups(P > 0.05), while FPH group were significantly higher than FM, PBPH, and SPH groups(P < 0.05). SOD, POD, LZM activities in serum of the FPH group were significantly higher than PBPH and SPH groups(P < 0.05), while FPH, FM, and YPH groups was not significantly different(P > 0.05). There were no difference in AKP, ACP, POD, SOD activities of hepatopancreas among FPH, YPH, and FM groups(P > 0.05), while FPH group were significantly higher than PBPH and SPH groups(P < 0.05). LZM and NOS activities in hepatopancreas of the FPH group were significantly higher than FM, PBPH, and SPH groups(P < 0.05), while FPH and YPH groups was not significantly different(P > 0.05). In conclusion, FPH had the best increasing tendency on growth and non-specific immunity compared with other diets, followed by YPH, PBPH and SPH.3. This study was conducted to investigate the effect of yeast protein hydrolysate on growth performance and non-specific immunity of juvenile pacific white shrimp(Litopenaeus vannamei). The experimental diets employed fishmeal, soybean meal, corn gluten meal, and shrimp meal as compound protein source, and formulated three kinds of isonitrogenous and isolipidic diets, which contained 0(FM), 1%(YPH-1), and 2%(YPH-2) yeast hydrolysate respectively. All diets were fed in triplicate groups of L. vannamei(initial weight 2.38±0.10 g) for 8 weeks. Results indicated that the feed intake in YPH-1 and YPH-2 groups was significantly higher than that in control group(P < 0.05).There were no significant impact on the final body weight, specific growth rate(SGR), and protein productive value(PPV) in all groups(P > 0.05), while feed efficiency ratio(FER) and protein efficiency ratio(PER) in YPH-1 and YPH-2 groups were significantly lower than those in control group(P < 0.05). The activity of serum AKP, ACP and hepatopancreas LZM, NOS in fish fed experimental diets were significantly higher than those in control group(P < 0.05). The activity of serum LZM, NOS and hepatopancreas AKP, ACP in YPH-1 group were significantly higher than those in control group(P < 0.05). The activity of serum SOD, T-AOC, POD, and hepatopancreas T-AOC, POD in YPH-1 group were significantly higher than those in control group(P < 0.05). The activity of hepatopancreas SOD in fish fed experimental diets were significantly higher than those in control group(P < 0.05). The crude protein of shrimp body in YPH-1 and YPH-2 groups were significantly higher than that in control group(P < 0.05), while there were no difference between YPH-1 and YPH-2 groups(P > 0.05). In conclusion, the study suggested that yeast protein hydrolyzate had none influence on growth performance with dietary 1%, 2% and improved the feed intake, non-specific immunity, and antioxidant capacity of juvenile pacific white shrimp. |
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