Effects Of Dietary Glucose Release Modes On The Utilization Of Nitrogen Nutrient In Piglets

The absorption and metabolism of nitrogen nutrient can be affected by the sources and compositions of dietary carbohydrate in livestock. Four kinds of glucose released dietary modes A、B、C、D based on secondary energy supply were built to investigate effects of different dietary modes on the utilization of nitrogen nutrient in piglets. The piglets were fed for 35 days and growth performances were determined during this period. The digestion and utilization of nitrogen were determined by nitrogen balance experiments in fourth day before the end of feeding trail. After 35-day feeding, piglets were euthanized and sacrificed at 2 、4 hours after morning or afternoon feeding, respectively. Then vena cava,portal vein blood and small intestine samples were collected to determine blood biochemical biomarkers、portal vein serum free amino acid content and mucosa nutrients transporter m RNA expression were determinated.The effects of glucose release dietary modes on nitrogen utilization were evaluated via growth performance, nutrient digestion, absorption and transport. This study aimed to provide scientific evidences for optimizing dietary energy compostion reasonably, increasing utilization efficiency of dietary nitrogen, and reducing emissions nitrogenous of organic matter.1 The resuLts of dietary glucose release rate in vitro assays showed that four groups glucose release dietary models(A、B、C、D)were successfuLly built. Slight difference was found between A and C groups, which presenced faster glucose release rate at incubation of 0~15mins, while glucose release rate of C group was significantly higher than that of A group(P<0.05) at 15 min, and decreased linearly between 30~120 mins. Glucose released were slow in B and D group, but the slow releasing period was from 60 to 90 mins in B group while from30 to 60 mins in D group.2 Dietary glucose release model couLd affect growth performance and nitrogen utilization in piglets significantly. ADG in B group was highest(P<0.05) while its F/G was lowest than that of other three groups. ME in A and B group was significantly higher than that of C and D group(P<0.05), there were no significantly between A, B and C, D, group respectively. ADE in B group was significantly higher than that of groups A, C, D(P<0.05), But didn’t differ significantly difference among A, C, D group(P>0.05).The reduction of fecal and urinary nitrogen and improvement of nitrogen apparent digestibility were significant in B group(P<0.05).3 Dietary glucose release models had effects on vena cava serum biochemical markers and free amino acids of piglets. TP in group B was significantly higher than that of other threegroups(P <0.05). Serum UREA levels in B, D group were significantly lower than that of A, C(P<0.05)group. There was no significant difference on blood glucose levels among the four groups(P>0.05). Total free amino acids in vena cava serum of B, D group were significantly higher than that of A, C group(P<0.05), Except Gly, the content of other free amino acids among four groups was significantly different(P<0.05).4 The resuLts of portal vein blood glucose and free amino acid levels showed that dietary glucose release model significantly affected the absorption of dietary glucose and amino acids in piglets. After intaking feed,the level of venous blood glucose and content of total and single free amino acids changed(P<0.05).5 Dietary glucose release models effected on glucose and amino acid transporter mRNA relative expression of piglets in intestinal mucosa. The abundance of SGLT1, B0AT1,EAAC1 mRNA in group B were significantly higher than those in groups A, C and D in posterior section of jejunum of the piglets(P<0.05); The m RNA expression of CAT1 among the four groups showed a downward trend from anterior to posterior section of small intestine of piglets.