High Carbon Storage Family Selection Of Betula Platyphylla Suk.

Forestry Carbon Sequestration is activities that people who use forest carbon storage function to absorb and fix atmospheric carbon dioxide combining carbon sequestration transaction through the implementation of afforestation and forest management and other measures. Forestry Carbon Sequestration attracts more and more attention in recent years because it plays an important role in solving the crisis of forest resources, reducing greenhouse gas concentrations in the atmosphere to mitigate global warming. Currently, studies on forest carbon sinks concentrate in the area of forest carbon sink capacity evaluation. However, tree selective breeding and genetic improvement research in order to increase forest carbon sinks are relatively lacking. Taking Birch (Betula platyphylla Suk.) diallel mating progeny as research objects and combining ability, breeding value as the key indicators, we selected high carbon storage family, parents and offspring and analysed related genes of carbon fixation. And we hoped to provide good materials and theoretical basis for selection, promotion and plantations of high carbon sequestration of birch varieties.In this study, DBH, biomass and carbon content was measured to obtain the whole forest DBH, organs (stem, bark, branch, leaf and root) and whole plant biomass and carbon storage. We analysed the genetic variation and used modeling to predict biomass and carbon stocks in each family. And we selected high carbon storage family, parents and offspring. Then we obtained six 1-year-old birch seedlings using Tissue Culture Techniques of asexual propagation of select tree and measured their growth and photosynthetic parameters. Finally we measured gene expression of BpRBCSlA, BpRBCS3B, BpGO, BpRCA of each family by using real-time PCR technology and analysed the correlation in conjunction with the photosynthetic parameters. Hoping to obtain birch with high carbon sequestration capabilities.The main research results are as follows:1. There was a significant difference among different families of Betula platyphylla, the first six families which had max diameter were 617, 619、642、606、634、623.2. Genetic variation of underground biomass and carbon stocks was higher and genetic variation of DBH and carbon content was smaller. Carbon content allocation in different organs was in order of leaf> bark> branch> stem> root and biomass and carbon storage allocation was root> stem> branch> leaf> bark.3. Biomass prediction model expression of the whole plant was B= 0.6328D16155and carbon storage prediction model expression was C= 0.30158D152880.4. With combining ability and breeding value being selection standard, high carbon storage families were:617,619,642,606,643,634; the first five fine individuals were 637-3-13,617-3-1,617-1-11,617-2-12,620-2-15; good parent was No.8; superior hybrid combinations were 8X11,5×15,14×5,15×11.5. There was a significant difference among gene expression of BpRBCSlA, BpRBCS3B, BpGO, BpRCA of different families. And there was a significant positive correlation between BpRBCS1A, BpRBCS3B and Pn, CUE, Gs.