Expression Vector Construction And Transformation Of Glyphosate Resistance Gene CP4-epsps In Sweet Potato

Sweet potato [Ipomoea batatas(L.) Lam] is one of important food crops and industrial crops. Its production is sufferring a lot from weeds damage, each year it can be cut down 5%-15% by weeds. The glyphosate resistant varieties was contained with the gene CP4-EPSPS transformed into sweet potato.In this study, the plant expression vector pCAMBIA1301-CP4-EPSPS was successfully constructed by connecting the cloning vector pMD18-T-CP4-EPSPS with the plant expression vector pCAMBIA1301 containing CaMV35 S promoter, NOS terminator, based on the amplification of gene CP4-EPSPS from a soybean variety with resistance to glyphosate. Then the stem section of sweet potato was infected with Agrobacterium tumefaciens harboring pCAMBIA1301-CP4-EPSPS recombinant vector assisted by vacuum infiltration method. It turned out that 5 of the 72 samples were identified to be positive by PCR verifivation, including 1 individual of Guangshu 87 and4 individuals of Fushu 18. Glyphosate tolerance identification results showed that 3individuals of Fushu 18 could tolerate glyphosate of 0.12% concentration. The above results was respected to facilitated breeding varieties with resistance to glyphosate and commercial production of sweet potato and proved that it was feasible to transfer the stem section with vacuum infiltration assisted Agrobacterium tumefaciens mediated transformation method.