Cloning And Bioinformatics Of TAC1 In Salix Psammophila And Characterization Of Its Tissue Expression

The branches development of trees plays an important role in plant morphogenesis, which affects the spatial structure of the whole trees, and which has an important impact on the efficiency of photosynthesis, tree biomass and garden landscaping, etc. With obvious branch angles of Salix psammophila, it can form a variety of trees spatial structure such as upright、standards andpillar, etc, it is ideal woody germplasm for finding gene resources which could influence tree’s spatial structure.The Salix psammophila was taken as the material in this study, and TAC1 gene was cloned. Bio informatics analysis, phyyogenetic tree and tissue specific expression of TAC1 gene were finished. The results are as follows:1. Cloning and bio information of SpsTACl. Though the homologous cloning technology to obtain the gene of SpsTAC1, The full-length cDNA of TAC1 genes were 819bp, encoding 273 amino acids and molecular weight of 65269.3Da, with theoretical isoelectric point of 5.16. The protein had no transmembrane domains. TAC1 was located in the cytoplasm by analyzing for subcellular localization.2. Amino acid homology of different plants and phylogenetic tree analysis of TAC1 showed that they have 5 same conserved regions of TAC1.3. The tissue specific expression of TAC1 genes. RT-PCR was used to analyze the expression levels of TAC1 genes among different tissues, RT-PCR assays indicated that TAC1 is mainly expressed in stem of salix, the second is the axillae, and the lowest expressed in root.The study obtained the gene of SpsTAC1 which has an important relationship with branch angles. This study may provide references for further research on the functional characteristics of TAC1.