| Cotton is one of the major textile industry raw materials, has important economic value, but cotton production vulnerability to the harm of plant diseases and insect pests, which causing great a serious of economic losses and costed yield and quality.Cotton aphid is one of the world pests of cotton by sucking mouthparts eat plant phloem of nutrients, when feeding plant fluids at the same time can also spread through saliva plant diseases.For pest prevention and control of the mainly depends on chemical pesticide, the chemical control is not only conducive to environmental protection, and as with the increase of number of use pests will produce resistance to its.Therefore, cultivating high quality of insect-resistant cotton has been the goal of breeding home. So this topic-cotton aphid with cotton as the research object, first of all, four varieties of cotton aphid resistance were studied.On this basis, through bioinformatics analysis, using the method of RACE separation of cotton GhRac6 genes were identified.The specific research results are as follows:1.Both in the selective examination and nonselective experiment, on the insect after 24 h,48 h,72 h, cotton aphid feeding tendency of Hong ye cotton is the weakest, therefore, preliminarily concluded that Hong ye cotton has strong resistance to aphid.2.The control and treatment of 4 kinds of cotton soluble sugar content change in size as follows:Zhong zhi cotton>Hong ye cotton>Chang guo hong ye cotton> xuzhou 142.When four cotton on cotton aphid after 48 hours, the soluble sugar content in the body is the biggest change of xuzhou,142, the smallest is Hong ye cotton, cotton aphid and its content changes in front the test results are not completely consistent, nonselective and selective sperm in cotton aphid in different varieties of development period and population dynamic experiment also found that the discretion of the soluble sugar content does not affect the growth of the aphids.This conclusion can also be used wang xing-ya’s point of view to explain, namely:the aphid damage soybean leaf soluble sugar content of soybean and the there was no significant difference is due to the violation aphids feeding sugar, soybean plants increased the amount of soluble sugar and aphids take food intake reached a balance, so the change is small.3.The control and treatment of 4 kinds of cotton SOD content change in size as follows:Zhong zhi cotton>Hong ye cotton>Chang guo hong ye cotton> xu zhou 142.The conclusion from the previous pick cotton aphid after 48 hours, the test results of nonselective and selective is not completely consistent, this is because the activity ofSOD is light, temperature, and other factors, the effect of SOD enzyme regulation mechanism is complex, Cu/zin-SOD, Mn-SOD gene expression and its enzyme activity change trend is not entirely consistent, that there are other genes involved in regulation of enzyme activity.4.48 hours after 4. Pick up cotton aphid control and processing of 4 kinds of cotton leaf callose relative to the increase of size:xu zhou 142>Zhong zhi cotton>Chang guo hong ye cotton>hong ye cotton.Xuzhou 142 with red leaves short conclusion with the cotton aphid in front of the selective and nonselective test conclusion showed consistency, often in the red leaves of cotton and cotton relative increase from the previous aphis gossypii selective and nonselective test conclusion inconsistencies, this is due to the resistance mechanism of cotton is complex, single callose index does not represent the strength of the insect-resistant.5. Cloned GhRac6 gene, the gene by 636 bp base composition, molecular weight of 23338.97 D, isoelectric point 6.718, encoding 211 amino acids, and its amino acid sequence homology analysis Cotton gene GhRac6, RAC phylogenetic tree showed that gene GhRac6 and Gossypium hirsutum ADY68837.1 are closest relative.At the same time to build the plant expression vector pMD-GhRac6 carrier, GhRac6 function for subsequent research laid a good foundation. |
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