Exploration Of Punch Biopsy For Canine Vital Oragans Through B-Mode Ultrasonography

A ultrasound-guided punch biopsy for internal organs (kidney, liver, spleen in this case), is generally an minor traumatic examination for pathology analyze by using biopsy needle to extract certain number of splanchnic tissue. The biopsy samples have its diagnostic value and making great contribution to build the relation between clinical symptom and pathomorphism. Furthermore, the pathological mechanism provides direction for future treatment and prevention of focal lesions. Compared with other kinds of biopsy methods, the ultrasound-guided biopsy technique not only offers a better performance on surgical accuracy but also has more advantages with simpliness and safety. In China, the application of biopsy through ultrasonography in small animal clinical practice lags behind. Here trials of biopsy for canine kidney, liver and spleen ultrasound-guided were carried out with fine-needle aspiration and biopsy needle to confirm its application possibility.After general anesthesia, ultrasonography was carried on dogs for determination of the best aspiration location, combining color doppler flow image in order to protect macro-vascular from injuries. The fine needle aspiration implemented for cytological examination. After making small incision in the skin, punch biopsy was done using a biopsy needle. The wax slides were cut from the biopsy samples, followed by hematoxylin-eosin staining and photography with a microscope.The results showed that biopsy was successful with both fine needle aspiration and the biopsy needle, and the biopsy samples offered the normal histologic structure of liver, spleen and kidney. In the microscopy of dog liver, there is not clear isolation between hepatic lobule and portal area because of that the hepatic lobule connective tissue of dog usually has poor development. The shape of liver cell nucleus is large and round. Within the hepatic lobule, the liver cells gather in the center of the central veins and arranged radially. The hepatic cords are in alignment and the blood sinusoids is highly classify. Within the portal area, there is interlobular vein, and the lumen is large and irregular; the interlobular arteries have small lumen and thick wall; the simple cubical epithelium of interlobular bile duct wall neatly arranged. In the case of spleen sample, there are clear boundary between the red pulp, white pulp and the marginal zone. The pulp veins is huge and clear in the red pulp, and parts of the red pulp are in congestion. The biopsy samples of kidney clearly figure the renal corpuscle and kidney tubules in the cortex. The body of the renal corpuscle is a sphere, and there is a curved capillary in the renal corpuscle. There are two layers of cysts wall formation of capsular space outside the renal corpuscle and contact to the proximal convoluted tubule. The proximal convoluted tubules have crude caliber and small, irregular lumen, and the epithelial cells of tube wall have indistinct cell borders.. The cavity inner surface have brush border. The distal convoluted tubule lumen is large and regular, and the epithelial cell of tube wall is a cube shape. There is no brush border on the cavity inner surface.In conclusion, by the aid of ultrasonography, the biopsy trials of canine kidney, liver, spleen was successful with both fine needle and biopsy needle, which was confirmed by cytology and histology assays, and that further defines this method is feasible and safe.