Functional Analysis Of VPS29 And VPS26 In Magnaporthe Oryzae

Rice blast, spreading all over the world, is one of the mainly disease of rice. The mycelium growth, conidiation and appressorium formation of Magnaporthe oryzae had been investigated for decades, however, material transporting in these metabolisms of Magnaporthe oryzae still need to take more investigations. Vps26 and Vps29 two proteins of cargo-selection subcomplex of retromer complex are involved in retrograding transport from endosomes to the trans-Golgi network. Deletion mutants of VPS29 and VPS26 in Magnaporthe oryzae showed no difference in mycelium growth rate, while these mutants exhibited mycelium growth defect in medium with exogenous Congo red, flourescent brightener and SDS. Conidia of AVps29 and AVps26 became one third of wild type, while spore morphology, germination and appressorium formation develop normally. AVps29 and AVps26 still infecting onion, however infection rate of deletion mutants only 5%, declined dramatically, compare with wild type. The distinct defect was pathogenicity decline in AVps29 and AVps26 in rice and barley. These results suggest that VPS29 and VPS26 involve in many metabolisms such as keeping cell wall integrality, mediating conidiation and having a pivotal role in cell wall integrity and pathogenicity.