The Study Of Violate Compounds And Flower Pigments In Chimonanthus Praecox L

Chimonanthus praecox L. (wintersweet) is a traditional fragrant plant that originates from China. It blooms in winter and gives strong and pleasing fragrance. Most wintersweet have yellow tapals, while some varieties harboring red inner tapals. For its high economic values, wintersweet is widely applied in gardens and used as the source of natural essential oil and scented tea.In this paper, aromatic compounds and color were analyzed in Chimonthus praecox. The method of HS-SPME-GC-MS was applied to determine the aromatic compounds in flowers of 9 C. praecox samples, in order to test the differences between clones and tapals and dynamic changes. Besides, flower pigments were qualitative and quantitative analyzed by HPLC and HPLC-QTOF-MS. The main results were as follows:1.70 volatile substances were identified in 9 C. praecox, most of which were terpenoids and benzenoids. Linalool, P-ocimene, benzyl acetate and methyl salicylate dominated the scent of wintersweet, which varies in different clones and had no relationship with color of inner petals. Besides, the difference between lu-2-5 and other 8 clones indicated that linalool is one of the important components of wintersweet.2. The dynamic changes of volatile compounds showed that less flavoring substances but more kinds of sesquiterpenoids exist in the flowers that didn’t open. As flowers open, volatile constituents decreased, while total content increasing.3. There were no differences of main aromatic compounds in medium and inner tepals, while latter and medium tepals of buds had more kinds of sesquiterpenoids. Besides, benzenoids increased in whole flowering stage of wintersweet, but less in red tepals than yellow ones.4.3 flavonols and 2 anthocyanins were detected in red tepals of C. praecox, nevertheless, there were only 3 flavonols in yellow ones. Contents of flavonols in medium tepals were more than inner tepals.5. The increment of benzenoids of red inner tepals in early opening stage was lower than those in yellow ones. However, its anthocyanins added at the same time. What’s more, benzenoids and anthocyanins derive from the same biosynthetic pathway. As a result, it was supposed that there’s an substrate competition relation between benzenoids and anthocyanins.