Study On The Regeneration System Of Sorbaria Kirilowii

Sorbaria kirilowii is a beautiful summer flowering shrub widely planted in northern China, with various high values in urban landscaping, ecological rehabilitation, medicine and food manufacturing. However, its single white flower color and plant materials containing harmful volatile components, have seriously limited its ornamental usage.This research focused on exploring the effects of various factors on in vitro regeneration process of Sorbaria kirilowii by leaves, stems and seeds, in order to establish a viable regeneration system of Sor-baria kirilowii, and lay the foundation for transgenosis research on ornamental value improvement of Sorbaria kirilowii. The main results were as follows:Callus could be induced from leaves, stems and seeds of Sorbaria kirilowii. While callus only in-duced from leaves and seeds had possibilities to regenerate to adventitious buds. Immature seeds were more suitable for regeneration than mature seeds.The suitable condition for callus induction of leaves was inoculated to WPM with 2,4-D 1.0mg/L and TDZ 0.1mg/L. The suitable condition for callus induction of seeds was inoculated to MS with 2,4-D 2.0mg/L and TD Z 0.1mg/L.The suitable differentiation medium for adventitious buds from leaf callus was WPM with TDZ 0.1mg/L,6-BA 0.5mg/L, NAA O.lmg/L, IBA 0.5mg/L, glucose 40g/L and agar 4g/L. The highest callus differentiation rate was 30.75%. The suitable medium for seed callus differentiation was 1/2MS with TDZ 0.3mg/L,6-BA 1.5mg/L, NAA 0.05mg/L and IBA 0.3mg/L. The highest differentiation rate was 45.80%.The method of reducing the vitrification problem of the regenerated plantlets is to add sucrose 40g/L and agar 8g/L in medium. The suitable medium for the regenerated seedling was WPM with NAA 0.1mg/L, banana 100g/L, sucrose 30g/L and agar 6g/L. The suitable culture medium for the prolifera- tion of the regenerated plantlets was MS with 6-BA 1.0mg/L, NAA O.lmg/L, sucrose 30g/L and agar 6g/L. The suitable medium for rooting was 1/4MS with NAA O.lmg/L, IBA 0.5mg/L, sucrose 30g/L andagar 6g/L. The suitable method for transplanting was to getting acclimatization of 10 days, and then transplanting the plantlets to peat with vermiculite 1:1 volume proportion of the mixed matrix. The highest survival rate was achieved at 85.60%.