The Functional Analysis Of Interaction Proteins Of Magnaporthe Oryzae Serine Protease MoSp1 In Rice

Rice(Oryza sativa) is one of the most important grain crops worldwide, however, rice blast (Magnaporthe oryzae) causes a main disease on rice, leading to nearly 10% loss. Our former study found that the secreted serine protease MoSpl in rice blast fungus can be a potential pathogenic factor. Two rice proteins (OsMb11 and OsZfp1) was identified by yest two hybrid to interact with MoSpl. The OsMb11 is Jacalin-like lectin domain protein, and the OsZfpl is C3HC4 type RING finger protein, according to bioinformatic predictions. So far, functional study on plant lectin and C3HC4 RING finger in rice against M. oryzae is rare, the mechanism is still unclear.In this study, we detected the transcriptional change of rice genes encoding these two proteins when inoculated with rice blast strain Guy11. The results showed that the expression level of OsMBL1 increased to the peak with 50 folds at the first 24 hour after spore-spaying, while the summit of OsZFP1 came 6 hour earlier with 3.8 folds, which revealed that both genes may respond to M. oryzae invasion. The overexpression of OsMBLl enhanced rice immunity against M. oryzae as well as OsZFP1. The statistic analysis indicated that the improvement of resistance against M. oryzae went as the elevation of OsMBL1 transcriptional level, however the relation between expression level and pathogenic index was unclear in OsZFP1 plants because of not enough lines.All of above, the overexpression of two MoSPl interaction genes, OsMBL1 especially, can protect rice from being attacked by M. oryzae.