Mitochondrial C3HC And C2H2 Type Zinc-Finger Proteins(ZFC3、ZFC2): Function And Molecular Mechanisms Involved In The MoLon1-mediated Disease Pathway In Magnaporthe Oryzae

Rice blast is one of the most important disease in the rice production, which brought huge losses to the rice yield. Magnaporthe oryzae caused the rice blast disease and was seen as a model filamentous fungi. The process of growth, the incidence and the epidemiological characteristics of this pathogenic fungi are well known by people. Breeding and chemical pesticides are the best ways to control the rice blast, but now it become more difficulty for the reason of its easy changing of M. oryzae. With the completion of genome sequencing, to explore the molecular pathogenic mechanism and molecular developmental mechanism has the potential scientific value and significance.As we all know, Mitochondria serve as the major source of ATP production in eukaryotic cells and have its own mt DNA which encoding a few of proteins. With in-depth study, Mitochondria are often involved in the pathogenicity in plant fungal pathogens, but the specific pathogenic mechanism is not clear by scientist. In the previous research in our lab, we found the mitochondria proteins partly mediated by Mitochondrial ATP-dependent Lon Protease(MAP1). Mitochondrial C3 HC and C2H2 type zinc-finger proteins(ZFC3、ZFC2)are mediated by MAP1. Therefore, elucidating the function and role of the zinc-finger protein is important for enriching the knowledge of mitochondrial transcription regulation machinery.ZFC3 and ZFC2 are interacting with Lon protease, Lon protease has important role in coping with multi-stress conditions. For the reason of ZFC3/2 are mediated by MAP1, we decide to study the function of the two proteins. Firstly, we adopt the methods of agrobacterium tumefaciens mediated transformation to knock out the ZFC3 and ZFC2. Meanwhile to find out where the two proteins are localized by fusion fluorescent protein. Then we carried on analysis of biological characteristics between WT and knock-out mutants. The △ZFC3 knock-out mutant grew at a faster rate as the wild type by measuring colony sizes, the production of conidia has increased significantly, and produced higher pigmentation in liquid and solid media. The results also showed that △ZFC3 didn’t sensitive to H2O2, CR and SDS. The △ZFC3 mutant showed ahead of time in the inoculation experiment of rice sheath cells and spray inoculation of rice seedlings. The △ZFC2 knock-out mutant grew at a same rate with wild type, but the production of conidia was lower than wild type.Above all, ZFC3/ ZFC2 regulate the proliferation and development of in M. oryzae,involved in a series of metabolic and regulatory process.