Development Of New EST Primer Based On Wheat Chromosome 2B And Genetic Analysis Of Resistance To Stripe Rust In A Tritielytrigia Introgression Line

Comparative genomics with Brachypodium distachyon, Oryza saliva and Zea mays to develop new molecular marker used in gene fine localization, gene clone and molecular marker assisted breeding, which own practical and theoretical value to research and application of wheat fine genes. Now the wheat stripe rust has become a danger to wheat production. And many resistant materials or varieties lost their resistance in face of Puccinia striiormis variation. Identification and research of stripe rust resistance genes in new resources is a long-term and urgent task. This study via method of comparative genomics, did sequence blast between wheat chromosome 2B and other three plant, Brachypodium distachyon, Oryza sativa and Zea mays, studied the homologous relation between the chromosome 2B and the three genomes, and designed new EST primers according to the collinearity segment on Brachypodium distachyon chromosome Bdl. Furthermore, this study did identification and genetic analysis to a new material 11B666-3. The results show that:1 Blast of the EST sequence on chromosome 2B and other three genomes show that there are 1125 homologous locus between them, and 287 in Zea mays,299 in Oryza sativa,539 in Brachypodium distachyon. Chromosome Bdl, Bd5, Os7, Os3 and Zm7 are high homologous with wheat chromosome 2B.2 Found that 13467797bp-17757616bp of chromosome Bdl focus 70 homologous locus. We thought that this section is high homologous with some segment of wheat chromosome 2B.3 According to the collinearity segments on chromosome Bdl, download and screened of its genes, and designed 242 pairs of EST primer, finally,162 of them were successfully done chromosomal localization on wheat chromosome by using nullisomic Chinese spring, and 38 pairs have amplification sites on wheat chromosome 2B.4 Study analyzed amplification to 162 pairs of primers which located on chromosome 2B, and found that primer’s effective rate is between 59.2% and 88.2%, and the average is 72.0%.105 pairs of primers are useful in material 11C666-3. Most of the primers can be used to different wheat group of molecular marker technology.5 Using disease-resistant wheat material 11C666-3 and two susceptible varieties, MY11 and Tai29, did their reciprocal cross and F1 self-cross, finally, observed F2 population resistance to Puccinia striiormis physiological race, and results show that F2 resistance separation ratio conforms to 3:1. So we concluded that 11C666-3’s resistance to stripe rust is control by a dominant gene in cell nucleus.