| Mycotoxins are a series of secondary metabolites of moulds, which have caused huge loss of animal husbandry. The present study was conducted to investigate the effect of natural mycoxoxins-contaminated corn on weaned piglets and the detoxification effect of a nutritive additive on it. The dietary treatments were arranged in a completely randomized 2x3 factorial design. A total of 36 (Duroc x (L andrace* Yorkshire)) piglets weaned at 26 days (initial weight 8.57 ± 0.21kg) were allotted to 6 dietary treatments (6 replicates per group and 1 piglet per replicate, pigs were penned singly) and fed with the diet that contained different levels of moldy corn (0,50%, 100%) and nutritive additive (0,0.2%). The trial lasted for 30 days. Precaval vein blood was collected from each pig before they were fed at day-15 and day-31 of the trial, serum sample was used to analyze glutamic-pyruvic transaminase (GPT), glutamic-oxalacetic transaminase (GOT), creatinine (Cr), cystatinC (CYS-C), etc. At the end of the trial, pigs were killed and liver, kidney and spleen were weighed to calculate organ index. Besides, liver samples were collected to analyze the amount of GPT, GOT and the mRNA expression of Bax, Bcl-2, IL-6, TNF-a,etc.(1) Mycotoxins had a significant effect on growth performance of weaned pigs. Final weight、ADG and ADFI of pigs from the 100%-mycotoxin-contaminated-corn groups (100%-MCC) were lower than those of normal-corn groups (NC) (P<0.05). Compared with NC, ADG and ADFI of pigs in 50%-MCC groups, and F/G of pigs in 100%-MCC groups tended to reduce. Nutritive additive and its interaction with mycotoxins had no effect on growth performance of pigs.(2) Liver index of pigs in 100%-MCC groups was higher than that of NC groups (P<0.05),so as the kidney index of pigs in 50%-MCC groups. There is trend that kidney index of pigs in 100%-MCC groups higher than that of NC groups. Nutritive additive and its interaction with mycotoxins had no effect on organ index.(3) The 15th-day serum GOP activity of pigs in 100%-MCC groups was significantly higher than that of 50%-MCC groups (P<0.05) and NC groups (P<0.01). The same index in 50%-MCC groups was higher compared to NC groups (P=0.08). The 15th-day and 31st-day serum GPT activity of pigs in 100%-MCC groups was significantly higher than that of NC groups (P<0.05) and 50%-MCC groups (P<0.01). GPT activity in liver of pigs in 100%-MCC groups was lower than that of NC groups (P<0.05). Nutritive additive and its interaction with mycotoxins had no effect on GOT and GPT activity in sreum and liver.(4) The 15th-day serum Cr concentration of pigs in 100%-MCC (P<0.01) and 50%-MCC (P<0.05) groups was significantly higher than that of NC groups. The 31th-day serum Cr concentration of pigs in 100%-MCC groups was higher compared to that of NC groups (P<0.05). The 15th-day serum CYS-C concentration of pigs in 100%-MCC (P<0.01) and 50%-MCC (P<0.05) groups was notably higher than that of NC groups. Nutritive additive and its interaction with mycotoxins had no effect on serum concentration of Cr and CYS-C.(5) Nutritive additive increased activity of SOD in the liver (P=0.099). Nutritive additive and its interaction with mycotoxins had no effect on activity of MDA and SOD in liver.(6) The 31st-day serum lysozym activity of pigs in 100%-MCC groups was lower compared to that of 50%-MCC and NC groups (P<0.05). Nutritive additive increased the 31st-day serum lysozym activity (P<0.05) and C4 concentration (P=0.072).(7) 100%-MCC significantly increased the mRNA expression of Bax, Bcl-2 (P<0.01) and Ape-1 in liver (P<0.05), and decreased mRNA expression of TNF-α in liver (P<0.05). Nutritive additive significantly increased mRNA expression of Bcl-2 in liver (P<0.01), and decreased mRNA expression of TNF-α, Ape-1 and IL-1β(P<0.0.1) in liver. The interaction of nutritive additive and mycotoxins had a significant effect on mRNA expression of the mentioned genes (P<0.05).The results suggested that mycotoxins decreased growth performance, caused oxidative stress and inflammatory response and impaired liver and kidney function of weaned pigs. Those effection were strengthened as the proportion of mouldy corn increased. Nutritive additive could repair the damage of the liver and reduce liver inflammation to some extent, caused by low concentration of mycotoxins in the diet. |
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