| Lipopolysaccharide (LPS). a component of the cell walls of gram-negative bacteria, causing inflammation response is the same as the gram-negative bacteria infection. Female reproductive tract directly open outside, therefore, it’s easier to be infected than internal organs. Female reproductive tract infection triggered a series of reproductive dysfunction, and then reduced the animal reproductive productivity. The hypothalamus-pituitary-ovarian axis is a complete neuroendocrine regulation system, the secretion of female reproductive hormone to maintain normal reproductive function plays an important role. Some studies shows the effect of LPS on reproductive hormones, and its biological effect is closely related to their receptors. In this study, we first detect the expression level of TLRs in female rabbit respiratory and digestive organ, such as liver, spleen, uterus etc. To understand the innate immune mechanism of female rabbit organs; Sequently, to explore the expression level of reproductive hormone receptor like Progesterone receptor(PR). Progesterone receptor membrane component (PGRMC), Gonadotropin releasing hormone receptor (GnRHR), Luteinizing hormone receptor (LHR). and Estrogen Receptor beta-subunit (ER-β), under the inflammation response caused by LPS in the uterus, and revealing the LPS effect on reproductive hormone receptor, to clarify the theoretical basis for reproductive disorders caused by LPS.36 health dieostrus female New Zealand White rabbits (5-6 months of age),4 non-treated rabbits are as Oh (n=4),16 rabbits in LPS-group were injected with LPS (0.5mg/Kg, n= 16). and 16 in the control group were injected with saline (LPS carrier). The tissues of lung, trachea, intestine, stomach, liver, spleen, uterine horn and body, cervix, ovary, oviduct and hypothalamus were collected in Oh rabbits to detect the expression profiles of TLRs. The tissue of uterus horn, uterus body and uterus cervix were collected at 1.5.3,6 and 12h of LPS or saline postinject to determine the expression lever of reproductive hormone receptors by real-time PCR and immunohistochemistrical assay (IHC assay).The results of expression profile of TLRs in various organs shown:(1) To compare with the liver, TLR2 had a higher expression in the trachea than other organs, and it had a relative low expression in the lung, intestine, stomach, spleen, ovary, and the hypothalamus (P<0.05); The expression level of TLR3 in the intestine and oviduct was greater than in other organs, and expression levels in the stomach and hypothalamus were lower than others (P<0.05); The expression level of TLR4 in the lung was greater than in other organs, and expression levels in the trachea, intestine, spleen, liver, ovary, hypothalamus and the oviduct were lower than others (P<0.05); TLR5 had a higher expression in the lung, trachea and stomach than other organs, and had a lower expression in the spleen (P<0.05); TLR6 had a higher expression in the lung, uterus horn and uterine body, and it had a relative low expression in the spleen and hypothalamus (P<0.05); The expression level of TLR8 in the lung and spleen was greater than others, and expression levels in the intestine, stomach, uterus horn, uterus body, cervix, ovary and oviduct were lower than the other organs (P<0.05); TLR10 had a higher expression in the trachea and spleen, and had a lower expression in intestine, stomach, uterus horn, uterus body, cervix, oviduct, hypothalamus and ovary (P<0.05).(2) LPS could significantly inhibit the expression of PR mRNA in the uterine horn and body, and cervix (P<0.05); However, the expressions of PR at protein level in endometrial epithelium of uterine horn and body, and cervix are not affected. The expression levels of PGRMC in the uterine body and cervix significantly decreased under the administration of LPS (P<0.05). Moreover, LPS could inhibit the pression of PGRMC at protein level in epithelium of cervcix (P<0.05). LPS could significantly change the expression pattern of GnRHR mRNA in the uterine horn and body, and cervix (P<0.05); But GnRHR expression had no significant differences of all groups in the uterine horn and body, and cervix (P<0.05).LPS could downregulate the expression of LHR mRNA in the uterine horn and body, and cervix (P<0.05). However, the expression of LHR at protein level in epithelium of the uterine horn and body, and cervix hadn’t significant difference between LPS and the control group. LPS could impact on the expression pattern of ER-β mRNA in the uterine horn and body, and cervix (P<0.05), and upregulate its expression at protein level in endmometrial epithelium of the uterine horn (P<0.05).Conclusion:The results decleared that TLRs had abundant expressions in the lung, trachea, intestine, stomach, liver, spleen, uterine horn and body, cervix, ovary, oviduct and hypothalamus. LPS could affect the expression of PR, GnRHR and LHR at mRNA level, and the expression of PGRMC and ER-β at protein level in the uterus, which is possible to lead to reproductive dysfunction of female rabbits in response to LPS. |
PDF Full Text Request